FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002294280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187722
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGC201781.6988823984063612No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG105990.892380540227427RNA PCR Primer, Index 47 (96% over 30bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG58510.4926236947703251No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGC56380.4746902052837281No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTG54390.4579354428056397No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTG48470.40809212930298505No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGA47190.39731519665376247No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG45340.38173916118418283RNA PCR Primer, Index 47 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG42240.3556387774243468RNA PCR Primer, Index 47 (96% over 30bp)
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGA37450.31530947477608395No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATG36090.3038589838362849No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA31380.26420323947859853No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAA28030.2359979860607112No Hit
ATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG26320.22160067759964033RNA PCR Primer, Index 47 (96% over 30bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTC25170.21191827717260436RNA PCR Primer, Index 47 (95% over 21bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTC24980.2103185762324854RNA PCR Primer, Index 47 (95% over 21bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTC23670.19928905922429657RNA PCR Primer, Index 47 (95% over 21bp)
CATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG23620.19886808529268632RNA PCR Primer, Index 47 (96% over 30bp)
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGA22160.18657564648966676RNA PCR Primer, Index 47 (96% over 30bp)
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACG20860.17563032426780004No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC20840.17546193469515592No Hit
CACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG19750.1662847029860523RNA PCR Primer, Index 37 (96% over 29bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG18830.15853878264442353No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTG18460.15542357555050762No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA17330.14590956469611574No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTC17320.14582536990979372RNA PCR Primer, Index 47 (95% over 21bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGA17100.14397308461070857No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCT15810.1331119571751639RNA PCR Primer, Index 47 (95% over 22bp)
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC15600.1313438666624008No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGA15360.12932319179067156No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGAT14930.12570281597882332No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATG14580.12275599845755152No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTG13030.10970580657763349No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACG13000.10945322221866734No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACAT12260.1032228080308355No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA12060.10153891230439446No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)