FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002294283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1521281
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG93850.6169142978844803RNA PCR Primer, Index 32 (96% over 33bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC92210.6061339095144158No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG37970.24959228439716263RNA PCR Primer, Index 32 (96% over 33bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC37390.24577970802238377No Hit
ACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG33660.22126089788802988RNA PCR Primer, Index 32 (96% over 33bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC25760.16933097830052435RNA PCR Primer, Index 32 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG23200.1525030549911555RNA PCR Primer, Index 32 (96% over 33bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC22050.14494363631702492RNA PCR Primer, Index 32 (95% over 24bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG21090.1386331650760116No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCA19260.12660382927282995No Hit
CATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG17520.11516610014849328RNA PCR Primer, Index 32 (96% over 33bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTC17310.11378568456452162No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCA17230.11325981196110384No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATT17170.11286540750854049No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC16090.10576612736240051RNA PCR Primer, Index 32 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)