FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002294287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610099
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGC70280.26926181727206516No Hit
GTTCTAAGTTGGTCGTTAATCGCCCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCCCAACCCGGTGCTGCGGGG64120.24566117990160527No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT34040.13041650910559333No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG33380.12788786938732977Illumina PCR Primer Index 2 (96% over 28bp)
GTAGGGCACTAGCCGGGCAACCGGCCGGCGCCCCGCAGCACCGGGTTGGGGACGCCCTTGGCAGGCTTCGGCCGT31000.11876944131237935No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGC28110.10769706436422526No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTCGTTAATCGCCCGCCGGACGGCCGAAGCCTGCCAAGGG26940.10321447577275804No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)