FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002294292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159672
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGC86550.7463317213832877No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG58620.5054877586076063RNA PCR Primer, Index 47 (96% over 30bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGC32190.27757848771031807No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTG23590.20341958760753043No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG23420.2019536558613125No Hit
ACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG23200.20005656771914818RNA PCR Primer, Index 47 (96% over 30bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG22310.19238198387130154RNA PCR Primer, Index 47 (96% over 30bp)
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTG20680.17832628536344758No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGA20310.17513572803344393No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGA16620.14331638601259666No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATG15310.13202008843879995No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA14410.12425927331176402No Hit
ATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG14280.12313826668230328RNA PCR Primer, Index 47 (96% over 30bp)
CATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG14280.12313826668230328RNA PCR Primer, Index 47 (96% over 30bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTC14260.1229658041239247RNA PCR Primer, Index 47 (95% over 21bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTC12770.11011734352472079RNA PCR Primer, Index 47 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)