FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5540
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC170730.812274368231048No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGT78314.133574007220219No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC56010.108303249097473No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1883.393501805054152No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC1532.7617328519855597No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1392.509025270758123No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG1182.1299638989169676No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG701.263537906137184No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG651.1732851985559567No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC540.9747292418772563No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT490.884476534296029No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT430.776173285198556No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA430.776173285198556No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTA420.7581227436823105No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT380.6859205776173285No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT380.6859205776173285No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG340.6137184115523465No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC300.5415162454873645No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGT280.5054151624548736No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT270.48736462093862815No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA270.48736462093862815No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG260.4693140794223827No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG210.37906137184115524No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC200.36101083032490977No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG180.3249097472924188No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATT170.30685920577617326No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC170.30685920577617326No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT160.2888086642599278No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA160.2888086642599278No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC160.2888086642599278No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG150.2707581227436823No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA150.2707581227436823No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG140.2527075812274368No Hit
ACACCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG140.2527075812274368No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC140.2527075812274368No Hit
CAACCAAAAGAACAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC140.2527075812274368No Hit
CAAGCAATCAAAGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGT140.2527075812274368No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT140.2527075812274368No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC130.23465703971119134No Hit
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG130.23465703971119134No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG120.21660649819494585No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT110.19855595667870035No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTG100.18050541516245489No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACG90.1624548736462094No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTGCATTCATCTCGT90.1624548736462094No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT90.1624548736462094No Hit
CAACCAAAAGAACAGCCTGTCTCTATACACATCTCCGAGCCCACGAGACG90.1624548736462094No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAAGCGTGTATCTCGT80.1444043321299639No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTTCCGAGCCCACGAGAC80.1444043321299639No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGCCCACGAGAC80.1444043321299639No Hit
CCTGACTCACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCG70.1263537906137184No Hit
CAAGCAATCAAGGCCAGTCTTTATACACATCTCCGAGCCCACGAGACGTG70.1263537906137184No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCTCACGAGACGT70.1263537906137184No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCGAGCCCACGAGACG70.1263537906137184No Hit
CAACCAAAAGACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACG60.10830324909747292No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCCCGAGAC60.10830324909747292No Hit
CCTGACTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGT60.10830324909747292No Hit
GTGGCTGCTCATCGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC60.10830324909747292No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCAC60.10830324909747292No Hit
CTTCAGTGGAGCATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA60.10830324909747292No Hit
CAAGCAAAAGAACAGCCTGTCTCTTTTACACATCTCCGAGCCCACGAGAC60.10830324909747292No Hit
CAACCAAAAGGACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC60.10830324909747292No Hit
CAACCAAAAGAACAGCCTGTCTCTTATATACATCTCCGAGCCCACGAGAC60.10830324909747292No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACACCTCCGAGCCCACGAGAC60.10830324909747292No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)