FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6754
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC214831.803375777317143No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCA113816.84927450399763No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC5858.661533905833581No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2012.97601421379923No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1942.8723719277465207No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG921.3621557595498963No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG801.184483269173823No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC670.9920047379330768No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC560.829138288421676No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG550.8143322475570033No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCAATCCTCATCTCGT480.7106899615042938No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA460.6810778797749482No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT420.621853716316257No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGT410.6070476754515842No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT410.6070476754515842No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC400.5922416345869115No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG380.5626295528575659No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG350.5182114302635475No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTA280.414569144210838No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG270.39976310334616527No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT270.39976310334616527No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT270.39976310334616527No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC250.3701510216168197No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG250.3701510216168197No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC240.3553449807521469No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA230.3405389398874741No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC210.3109268581581285No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT210.3109268581581285No Hit
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG180.26650873556411014No Hit
GTAGTGCTGCTCATCGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG160.23689665383476458No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCAAGCGATATCTCGT160.23689665383476458No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT160.23689665383476458No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGAC160.23689665383476458No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC150.22209061297009178No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAAGCGTGTATCTCGT150.22209061297009178No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGGACC140.207284572105419No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG140.207284572105419No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG140.207284572105419No Hit
CCTGACTCACATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCG140.207284572105419No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT130.19247853124074624No Hit
CAACCAAAAGAACAGCCTGTCTCTGATACACATCTCCGAGCCCACGAGAC120.17767249037607344No Hit
CAACCAAAAGAACAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC110.16286644951140067No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACAGACC110.16286644951140067No Hit
CTTCAGTGGAGCATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA110.16286644951140067No Hit
ACACCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG110.16286644951140067No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGAC100.14806040864672787No Hit
CAAGCAATCAAAGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCA100.14806040864672787No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAACC100.14806040864672787No Hit
CAACCAAAAGAACAGCCTGTCTCTTATATACATCTCCGAGCCCACGAGAC100.14806040864672787No Hit
ACATCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG100.14806040864672787No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT100.14806040864672787No Hit
CAAGCAATCAAGGCCAGTCTCTTATACAAATCTCCGAGCCCACGAGACCA90.13325436778205507No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCCACGTATATCTCGT90.13325436778205507No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA90.13325436778205507No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC90.13325436778205507No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGCGAC90.13325436778205507No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTTCCGAGCCCACGAGAC90.13325436778205507No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGGATAACAATCTCGT90.13325436778205507No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCCCCGAGCCCACGAGACCA80.11844832691738229No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG80.11844832691738229No Hit
GAAGCAGTGGATCTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCG80.11844832691738229No Hit
CAACCAAAAGAACAGCCTGTCTCTTATCACATCTCCGAGCCCACGAGACC80.11844832691738229No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCG70.1036422860527095No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAGAGACC70.1036422860527095No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACC70.1036422860527095No Hit
CTTCAGTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA70.1036422860527095No Hit
ATAGCAGAATCATCGTGGTCTCCTACAACTTCTGAAGGCTGTCTCTTATA70.1036422860527095No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGCCCACGAGAC70.1036422860527095No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGTCCACGAGAC70.1036422860527095No Hit
CAACCAAAAGAACAGCCTGTCTCTTAACACATCTCCGAGCCCACGAGACC70.1036422860527095No Hit
CAAGCAATCAAGGCCAGTCTGTCTCTTATACACATCTCCGAGCCCACGAG70.1036422860527095No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)