FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC41524.585308056872037No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG21012.440758293838861No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG1589.360189573459715No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG1217.168246445497631No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT875.154028436018957No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT623.672985781990521No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC563.3175355450236967No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC331.9549763033175356No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC331.9549763033175356No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT321.8957345971563981No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC311.8364928909952605No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT201.1848341232227488No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC181.066350710900474No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG171.0071090047393365No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT140.8293838862559242No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA140.8293838862559242No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG110.6516587677725119No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG110.6516587677725119No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG110.6516587677725119No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAA100.5924170616113744No Hit
GTTTCCAGGTCAAGGGTAACGGAACGTGGACCAAGTCCCGCGTCTCCTCC100.5924170616113744No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGGATAACAATCTCGT80.47393364928909953No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT70.4146919431279621No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG70.4146919431279621No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCAATCCTCATCTCGT70.4146919431279621No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT70.4146919431279621No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT70.4146919431279621No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTGCATTCATCTCGT60.35545023696682465No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCAAGCGATATCTCGT60.35545023696682465No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAAGCGTGTATCTCGT60.35545023696682465No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCTTG40.23696682464454977No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCG40.23696682464454977No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCCCCACGTTGC40.23696682464454977No Hit
GGTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTG40.23696682464454977No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCG40.23696682464454977No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCCACGTATATCTCGT40.23696682464454977No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCG40.23696682464454977No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG30.17772511848341233No Hit
CATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAA30.17772511848341233No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCG30.17772511848341233No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCAC30.17772511848341233No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT30.17772511848341233No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGTGTCTCCACGTTGC30.17772511848341233No Hit
GTACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG30.17772511848341233No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAAACCA30.17772511848341233No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC30.17772511848341233No Hit
ATCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC30.17772511848341233No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC30.17772511848341233No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC20.11848341232227488No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG20.11848341232227488No Hit
AGCCAAAGGGTCTAAAGAAGTGGAAGTCGCTCTTGTTCGGAAAGACAAAG20.11848341232227488No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGTACCG20.11848341232227488No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCG20.11848341232227488No Hit
TCTCGTGCTCGCAGATGCCACCCAGCGGCTCCGGGGCGGCAGGTGGGGCG20.11848341232227488No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGT20.11848341232227488No Hit
GTCATTGGCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC20.11848341232227488No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC20.11848341232227488No Hit
GTCATTGTCACTGGTCAGCTCCAACACCTTCTGCTGCGTCTCCACGTTGC20.11848341232227488No Hit
TTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGG20.11848341232227488No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA20.11848341232227488No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCG20.11848341232227488No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACAGACG20.11848341232227488No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGGCACCGGGTCATTCTTTT20.11848341232227488No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)