FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG22112.59977194982896No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG19711.231470923603194No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC1076.100342075256556No Hit
GTTTCCAGGTCAAGGGTAACGGAACGTGGACCAAGTCCCGCGTCTCCTCC925.245153933865451No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG864.903078677309008No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC834.732041049030787No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC583.30672748004561No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT533.0216647662485747No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT472.679589509692132No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC442.508551881413911No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT412.33751425313569No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT341.9384264538198404No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG321.8244013683010263No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT301.7103762827822122No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT301.7103762827822122No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA301.7103762827822122No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC281.596351197263398No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA281.596351197263398No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG271.5393386545039909No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATT241.3683010262257698No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT241.3683010262257698No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG241.3683010262257698No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG211.1972633979475484No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG211.1972633979475484No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT211.1972633979475484No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA191.0832383124287344No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG170.9692132269099202No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC160.9122006841505131No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGACACCGGGTCATTCTTTT110.6271379703534777No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC100.5701254275940707No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAAACCA100.5701254275940707No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCC80.45610034207525657No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGGCACCGGGTCATTCTTTT80.45610034207525657No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC70.3990877993158495No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCAC60.34207525655644244No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT60.34207525655644244No Hit
CCTGACTCACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG40.22805017103762829No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTG40.22805017103762829No Hit
CTGTTTTCATCCCACAGGTTGTATACGCATTGGCGGAAGTACTCAGAGGG30.17103762827822122No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG30.17103762827822122No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCCCCGGGGCGGCAGGTGGGGCG30.17103762827822122No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACACCATTCAATCTCGT30.17103762827822122No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCTGCT30.17103762827822122No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCCACGTATATCTCGT30.17103762827822122No Hit
TTTCAGGTCAGGGGTAACGGAACGTGGACCAAGTCCCGCGTCTCCTCCCG30.17103762827822122No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCCCCTCC30.17103762827822122No Hit
CATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAA20.11402508551881414No Hit
GTTTCCAGGTCAAGGGTAACGGACCGTGGACCAAGTCCCGCGTCTCCTCC20.11402508551881414No Hit
GTCATTGTCACTGGTCAGCTCCGAGCCCACGAGACCCACGTATATCTCGT20.11402508551881414No Hit
GCACAGGTCGTATACGCATTGGCGGCAGTACTCAGAGGGACTCAGCACCG20.11402508551881414No Hit
GCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCA20.11402508551881414No Hit
GTCCAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG20.11402508551881414No Hit
GTTTCCAGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCC20.11402508551881414No Hit
CTTCTTGTTTTCGGGACATGCTTCGGCCCCTAGCCCTCCTTGGTCACTGT20.11402508551881414No Hit
CTGTTTTCATCCCACAGGTTGTTATTTTGTACTTTGAGCCAAGCACATTT20.11402508551881414No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCG20.11402508551881414No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)