FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC3512.237762237762238No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG238.041958041958042No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC238.041958041958042No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG196.643356643356643No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG134.545454545454546No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG124.195804195804196No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT103.4965034965034967No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC103.4965034965034967No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC103.4965034965034967No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT93.146853146853147No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC82.797202797202797No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA72.4475524475524475No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT51.7482517482517483No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA41.3986013986013985No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG41.3986013986013985No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG41.3986013986013985No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT41.3986013986013985No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG41.3986013986013985No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT41.3986013986013985No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT41.3986013986013985No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACACCATTCAATCTCGT31.048951048951049No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCG31.048951048951049No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG31.048951048951049No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG31.048951048951049No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG31.048951048951049No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAA20.6993006993006993No Hit
GTAAAAGTTGAAATGTCAGGGCCCACGAGACACCATTCAATCTCGTATGC20.6993006993006993No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT20.6993006993006993No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC20.6993006993006993No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT20.6993006993006993No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGTCCACTTCTACAAT10.34965034965034963No Hit
GTTTCCAGGGCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCT10.34965034965034963No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATTTCCGAGCCCACGAGACAA10.34965034965034963No Hit
AACTGTAGCTTACACTGACTCCGAGCCAACGAGACACCATTCAAACTCGT10.34965034965034963No Hit
CAAGCAAAAGAACAGCCTGTCACTTATACACATCTCCGAGCCCACGAGAC10.34965034965034963No Hit
AACTGTAGCTTACACTAACTCCGAGTCCACGAGACACCATTCAAACTCGT10.34965034965034963No Hit
CTATTTGCTCAATGTCCGAGCCCACGAGACGGATAACAACTTCGTATGCC10.34965034965034963No Hit
GCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGCA10.34965034965034963No Hit
AGCCAAAGGGTCTAAAGAAGTGGAAGTCGCTCTTGTTCGGAAAGACAAAG10.34965034965034963No Hit
TCTCGTGCCCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG10.34965034965034963No Hit
AAAGTCTCTGAGGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA10.34965034965034963No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGACACCACAATGCTGGCGGGGACCG10.34965034965034963No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA10.34965034965034963No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGT10.34965034965034963No Hit
CTGGAATCCCGAGCCCACGAGACGATGCACAACATCGTATGCCGTCTTCT10.34965034965034963No Hit
CATCATAGCCAGATGCCCAGAGATTAGAGCGCATAACAAGTAAAGGACGG10.34965034965034963No Hit
CTCTCAGCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTC10.34965034965034963RNA PCR Primer, Index 32 (96% over 27bp)
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTG10.34965034965034963No Hit
GGGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCG10.34965034965034963No Hit
AAACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACG10.34965034965034963No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACACCATTCAAACTCGT10.34965034965034963No Hit
CCTGACTCACGTTTCCGAGCCCCCGAGACAACCAACGATCTCGTATGCCG10.34965034965034963No Hit
AACTGTAGCTTACATGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAA10.34965034965034963No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATT10.34965034965034963No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACTG10.34965034965034963No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAACCAACGAGCTCGT10.34965034965034963No Hit
CTTTGACGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTT10.34965034965034963No Hit
GTAGTGCTGCTCGACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGT10.34965034965034963No Hit
GTAAAAGTTGAAATGTCAGGGCCCACGAGACACCATTCTAGATCGTATGC10.34965034965034963No Hit
CTTTGACAGCACACGAGTTTCACCGGGATCACCAATGCCATACTCATTTT10.34965034965034963No Hit
ATAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCAC10.34965034965034963No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACACCATTCAATGTCGTATGCCG10.34965034965034963No Hit
CTTTGAAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTT10.34965034965034963No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACACCATTCAACCTCGTATGCCG10.34965034965034963No Hit
CATAGTTACCTTCTCCGAGCTCACGAGACACCATTCAATCTCGTATGCCG10.34965034965034963No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10.34965034965034963No Hit
ATAGCAGAATCATCGTGGTCTCCGAGCCCACGAGACAACCAACGAGCTCG10.34965034965034963No Hit
TGGACAAGAACAGCAACGAGCCCACGAGACCAATCCTCATCTCGTATGCC10.34965034965034963No Hit
CCTTTAACAAGTATGCCTGTCAGCATCACACCGGTGACCAGTCCTGAGGC10.34965034965034963No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCAATCCTGAGGGCGG10.34965034965034963No Hit
TGGACAAGAACAGCAACGAGCCCACGAGACGATGCACAATCTCGTATGCC10.34965034965034963No Hit
CTGGAATCCCGAGCCCACGAGACCAATCCTCATCTCGTATGCCGTCTTCT10.34965034965034963RNA PCR Primer, Index 18 (95% over 24bp)
GCACAGGTCATATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG10.34965034965034963No Hit
GGCTGTGGTGTGAGTCCGGGTGGCTGCTCATCGGGCTGTGTCTTATACAC10.34965034965034963No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTTCGAGCACACGAGAC10.34965034965034963No Hit
CCTGACTCACGTTTCCGAGCCCACGAGCCGGATAACAATCTCGTATGCCG10.34965034965034963No Hit
AAAGTCTCTGATGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA10.34965034965034963No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCCACGTATACCTCGT10.34965034965034963No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGTCATACTCATTT10.34965034965034963No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)