FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2373
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC68628.908554572271388No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGA38516.224188790560472No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2299.65023177412558No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG582.444163506110409No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG471.9806152549515381No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC451.8963337547408345No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC441.8541930046354824No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC331.390644753476612No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC311.3063632532659082No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG291.2220817530552044No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC251.053518752633797No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC251.053518752633797No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGT190.8006742520016856No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG170.7163927517909818No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG170.7163927517909818No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACACCATTCAATCTCGT150.6321112515802781No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT150.6321112515802781No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT130.5478297513695743No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC130.5478297513695743No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA110.4635482511588706No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT110.4635482511588706No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA100.42140750105351876No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG90.37926675094816686No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG90.37926675094816686No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT90.37926675094816686No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTA90.37926675094816686No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC80.337126000842815No Hit
GTAGTGCTGCTCATCGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG80.337126000842815No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGCCCACGAGAC80.337126000842815No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG70.2949852507374631No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT70.2949852507374631No Hit
CTTCAGTGGAGCATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA60.2528445006321113No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT60.2528445006321113No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG50.21070375052675938No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC50.21070375052675938No Hit
CTTCAGTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA50.21070375052675938No Hit
CAACCAAAAGAACAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC50.21070375052675938No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC50.21070375052675938No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGCGACGA40.1685630004214075No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT40.1685630004214075No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGAC40.1685630004214075No Hit
CAACCAAAAGAACAGCCTGTCTCTTATATACATCTCCGAGCCCACGAGAC40.1685630004214075No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAAGAGAC40.1685630004214075No Hit
CCTGACTCACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCG30.12642225031605564No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACACCATTCAAACTCGT30.12642225031605564No Hit
GCACAGGTCGTATACGCATCTCCGAGCCCACGAGACGATGCACAATCTCG30.12642225031605564No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACGTCTCCGAGCCCACGAGACGA30.12642225031605564No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACACCATTCAACCTCGTATGCCG30.12642225031605564No Hit
TGAATGAGGTCAAGAGGATCTGGAAATAAAACCCAAAACCCTGTCTCTTA30.12642225031605564No Hit
GTCATTGTCACTGGTCAGCTCCGAGCCCACGAGACACCATTCAATCTCGT30.12642225031605564No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGGACG30.12642225031605564No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGGACGAT30.12642225031605564No Hit
CCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT30.12642225031605564No Hit
CAACCAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACG30.12642225031605564No Hit
CAAGCAATCAAGGCCAGTCCCTTATACACATCTCCGAGCCCACGAGACGA30.12642225031605564No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACG30.12642225031605564No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCCCCGAGCCCACGAGAC30.12642225031605564No Hit
CAACCAAAAGAACAGCCTGCCTCTTATACACATCTCCGAGCCCACGAGAC30.12642225031605564No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGAC30.12642225031605564No Hit
CAAGCAAAAGAACAGCCTGTCTCTTGTACACATCTCCGAGCCCACGAGAC30.12642225031605564No Hit
CAAGCAATCAAAGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGA30.12642225031605564No Hit
GCACAGGTCGTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCG30.12642225031605564No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATTTCCGAGCCCACGAGACGA30.12642225031605564No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATATCCGAGCCCACGAGAC30.12642225031605564No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT30.12642225031605564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)