FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC100638.149412210845654No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC49118.6196435343193No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAA1565.915813424345847No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1074.057641259006447No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC913.450891164201744No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC180.6825938566552902No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG180.6825938566552902No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG150.5688282138794084No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC140.5309063329541145No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC130.4929844520288207No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGT130.4929844520288207No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG130.4929844520288207No Hit
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG120.4550625711035267No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT110.41714069017823285No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC100.37921880925293894No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA90.3412969283276451No Hit
CAACCAAAAGAACAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC90.3412969283276451No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC80.30337504740235116No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC80.30337504740235116No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT80.30337504740235116No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA80.30337504740235116No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATATACATCTCCGAGCCCACGAGAC70.26545316647705725No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACACCATTCAATCTCGT70.26545316647705725No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG70.26545316647705725No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC70.26545316647705725No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGGACA70.26545316647705725No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT70.26545316647705725No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA70.26545316647705725No Hit
CCTGACTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGT60.22753128555176336No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCATGAGAC60.22753128555176336No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTA60.22753128555176336No Hit
CTTCTTGTTTTGGGGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG60.22753128555176336No Hit
CAAGCAATCAAAGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAA60.22753128555176336No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAGAGACA50.18960940462646947No Hit
ACACCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG50.18960940462646947No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACA50.18960940462646947No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGAC50.18960940462646947No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGCCCACGAGAC50.18960940462646947No Hit
CAAGCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC40.15168752370117558No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC40.15168752370117558No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG40.15168752370117558No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG40.15168752370117558No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATTCCGAGCCCACGAGACA40.15168752370117558No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG40.15168752370117558No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACGTCTCCGAGCCCACGAGAC40.15168752370117558No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGCGAC40.15168752370117558No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAACCAACGATCTCGT40.15168752370117558No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGAC40.15168752370117558No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTTCGAGCCCACGAGAC40.15168752370117558No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT40.15168752370117558No Hit
ACATCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG40.15168752370117558No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGGGCCCACGAGAC40.15168752370117558No Hit
CAACCAAAAGAACAGCCTGTCTCTTTTACACATCTCCGAGCCCACGAGAC40.15168752370117558No Hit
CAAGCAAAAGAACAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC40.15168752370117558No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT40.15168752370117558No Hit
AAAGTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC30.11376564277588168No Hit
CAAGCAATCAAGGCCAGTCTCTGATACACATCTCCGAGCCCACGAGACAA30.11376564277588168No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT30.11376564277588168No Hit
CAACCAAAAGAACAGCCTGTTTCTTATACACATCTCCGAGCCCACGAGAC30.11376564277588168No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG30.11376564277588168No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGAC30.11376564277588168No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGACCACGAGAC30.11376564277588168No Hit
CAACCAAAAGAACAGCCTGTCTCTTTACACATCTCCGAGCCCACGAGACA30.11376564277588168No Hit
CCTGACTCTTATACACATCTCCGAGCCCACGAGACAACCAACGACCTCGT30.11376564277588168No Hit
ACACCAGCCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG30.11376564277588168No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCTACGAGAC30.11376564277588168No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCGAGCCCACGAGACA30.11376564277588168No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCCCGAGCCCACGAGACA30.11376564277588168No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTACGAGCCCACGAGAC30.11376564277588168No Hit
CAACCAAAAGAACAGCCTGTCTCTTAACACATCTCCGAGCCCACGAGACA30.11376564277588168No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG30.11376564277588168No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT30.11376564277588168No Hit
CAACCAAAAGAACAGCCTGTCTCTATACACATCTCCGAGCCCACGAGACA30.11376564277588168No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA30.11376564277588168No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC30.11376564277588168No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGTCCACGAGAC30.11376564277588168No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)