FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11783
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC439037.25706526351523No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCC201917.134855300008486No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC11299.581600611049817No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC4453.7766273444793343No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC3072.605448527539676No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCCACGTATATCTCGT850.7213782568106594No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGT660.5601289994059239No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA630.5346685903420182No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG600.5092081812781125No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG570.4837477722142069No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG520.4413137571076975No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC510.4328269540863957No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTA470.39887974200118814No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC450.3819061359585844No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG370.3140117117881694No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC370.3140117117881694No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT360.3055249087668675No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC350.29703810574556566No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT330.28006449970296193No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC320.27157769668166004No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA310.26309089366035815No Hit
CAAGCAATCAAAGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCC300.25460409063905626No Hit
ACACCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG300.25460409063905626No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT290.2461172876177544No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG280.2376304845964525No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG270.22914368157515064No Hit
GAAGCAGTGGATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG250.2121700755325469No Hit
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG240.20368327251124502No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG240.20368327251124502No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG230.19519646948994315No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC230.19519646948994315No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT230.19519646948994315No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC230.19519646948994315No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG220.18670966646864126No Hit
CAACCAAAAGAACAGCCTGTCTCTGATACACATCTCCGAGCCCACGAGAC220.18670966646864126No Hit
CAACCAAAAGAACAGCCTGTCTCTTATATACATCTCCGAGCCCACGAGAC220.18670966646864126No Hit
CAACCAAAAGAACAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC210.17822286344733937No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGAC200.16973606042603753No Hit
CTTCAGTGGAGCATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA190.16124925740473564No Hit
CCTGACTCACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG180.15276245438343375No Hit
GTAGTGCTGCTCATCGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG180.15276245438343375No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACC180.15276245438343375No Hit
CTTCAGTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA180.15276245438343375No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCGAGCCCACGAGACC170.14427565136213188No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTCCGAGCCCACGAGACC170.14427565136213188No Hit
CAAGCAATCAAGGCCAGTCTGTCTCTTATACACATCTCCGAGCCCACGAG170.14427565136213188No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT170.14427565136213188No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGGACC150.12730204531952813No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTTCGAGCCCACGAGAC150.12730204531952813No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACAGACC150.12730204531952813No Hit
CCTGACTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGT150.12730204531952813No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTTCCGAGCCCACGAGAC150.12730204531952813No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCGAGCCCACGAGACCCA140.11881524229822625No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCCCCGAGCCCACGAGAC140.11881524229822625No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGCCCACGAGAC140.11881524229822625No Hit
CAACCAAAAGAACAGCCTGTCTTTTATACACATCTCCGAGCCCACGAGAC140.11881524229822625No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGAC130.11032843927692437No Hit
CAACCAAAAGAACAGCCTGTCTCTTTTACACATCTCCGAGCCCACGAGAC130.11032843927692437No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCATGAGAC120.10184163625562251No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGACCACGAGAC120.10184163625562251No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAGAGACC120.10184163625562251No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCAAGCGATATCTCGT120.10184163625562251No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG120.10184163625562251No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)