FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002294330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365703
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG45031.231327060483507TruSeq Adapter, Index 6 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG21570.5898228890657172TruSeq Adapter, Index 25 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGC17900.48946822968365045No Hit
CATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG15430.42192708290607406TruSeq Adapter, Index 6 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG14000.3828243137190562TruSeq Adapter, Index 6 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG13930.3809101921504609TruSeq Adapter, Index 25 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG9960.27235215461727136RNA PCR Primer, Index 31 (96% over 29bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTC9380.2564922901917676No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTC8210.2244991154023894No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTC7680.2100064806687394No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGC7010.19168560279789884No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCGTC5340.14602013108998285No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)