FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226198
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG18970.8386457882032556RNA PCR Primer, Index 32 (96% over 33bp)
ACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG8600.38019787973368463RNA PCR Primer, Index 32 (96% over 33bp)
CATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG6720.2970848548616699RNA PCR Primer, Index 32 (96% over 33bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC6150.27188569306536753No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG5810.25685461409915206RNA PCR Primer, Index 32 (96% over 33bp)
ATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG5710.2524337085208534RNA PCR Primer, Index 32 (96% over 33bp)
CACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG4060.17948876647892553TruSeq Adapter, Index 8 (96% over 28bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC2340.10344919053218861RNA PCR Primer, Index 32 (95% over 24bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC2300.10168082830086915RNA PCR Primer, Index 32 (95% over 24bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC2270.10035455662737955RNA PCR Primer, Index 32 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)