FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002294335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1713873
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGC135480.790490310542263No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG46160.26933150822727236Illumina PCR Primer Index 2 (96% over 28bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGC45590.26600570754075714No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTC32900.19196288173044326No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTC30150.1759173521025187No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTC28590.16681516074995054No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGC27080.15800470629970834No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGC24190.14114231334527122No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGT23990.13997536573596758No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG21720.12673051037037167No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGT19910.11616963450617403No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTC19220.1121436652540766No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)