FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002294344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154775
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGC234261.0871668735714866No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGC86060.3993920478936316No Hit
CTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG67260.31214395934610345Illumina PCR Primer Index 10 (96% over 27bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTC66760.3098235314592011No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTC61520.2855054472044645No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTC56600.2626724367973454No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTC33750.15662888236590827No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCA31220.1448875172581824No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATC27720.12864452204986598No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG27070.12562796579689295Illumina PCR Primer Index 10 (96% over 27bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG26910.1248854288730842No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCA26840.12456056896891787No Hit
ACACATCTCCGAGCCCACGAGACATCATCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG23580.10943137914631458Illumina PCR Primer Index 10 (96% over 27bp)
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCATC22560.1046977062570338No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)