FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002294347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2378669
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGC97640.4104816601216899No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG52810.2220149167454572Illumina PCR Primer Index 2 (96% over 28bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGC39750.16711026208354338No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCGTC24500.10299877788797011No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)