FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147667
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG50483.418502441303744TruSeq Adapter, Index 1 (96% over 29bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGC29622.005864546581159No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC25921.7553007781020808No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC23531.593450127652082No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG21431.4512382590558486RNA PCR Primer, Index 1 (96% over 29bp)
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG21091.4282134803307442RNA PCR Primer, Index 1 (96% over 29bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC18741.2690716273778162No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG15451.0462730332437173TruSeq Adapter, Index 1 (96% over 29bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG10610.7185085360981127TruSeq Adapter, Index 1 (96% over 29bp)
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT9720.6582377917882803No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGC9480.6419850068058538No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC9460.6406306080573182No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC8850.5993214462269837No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC8630.5844230599930926No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG8320.5634298793907915RNA PCR Primer, Index 40 (96% over 30bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG8290.5613982812679881No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT7950.5383735025428836No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGA7640.5173803219405826No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG7190.4869063500985325No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT6770.4584639763792859No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG6730.45575517888221473No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA6620.44830598576526914No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT6610.44762878639100134No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG6540.44288839077112696No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA6440.4361163970284491No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT6230.4218952101688258No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG6040.40902842205773804No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG6040.40902842205773804No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT5750.38938964020397243No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC5550.37584565271861686No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT5420.3670420608531358No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG4760.3223469021514624No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG4210.28510093656673463No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG4160.28171493969539574No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG4060.27494294595271795No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG3980.2695253509585757No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT3900.2641077559644335No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGA3390.2295705878767768TruSeq Adapter, Index 1 (96% over 29bp)
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCT3320.22483019225690237TruSeq Adapter, Index 1 (95% over 21bp)
GTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG3220.21805819851422456TruSeq Adapter, Index 1 (96% over 29bp)
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACA3180.21534940101715347No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG2950.19977381540899455No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG2930.19841941666045904No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG2910.19706501791192346No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA2900.19638781853765566No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC2850.19300182166631677No Hit
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2800.1896158247949779No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT2760.18690702729790679No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT2740.1855526285493712TruSeq Adapter, Index 1 (95% over 22bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT2700.18284383105230012No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC2700.18284383105230012TruSeq Adapter, Index 1 (95% over 23bp)
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT2680.18148943230376455No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC2660.180135033555229No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA2580.17471743856108676No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA2560.17336303981255122No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT2520.1706542423154801No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC2500.16929984356694455No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA2490.16862264419267675No Hit
GTGTATAAGAGACAGAAAGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT2430.1645594479470701TruSeq Adapter, Index 1 (95% over 22bp)
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC2390.16185065044999897No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT2370.16049625170146345No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA2260.15304705858451786No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC2200.1489838623389112No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG2200.1489838623389112No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATC2200.1489838623389112No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG2170.14695226421610785TruSeq Adapter, Index 1 (96% over 29bp)
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA2150.1455978654675723No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT2110.14288906797050122No Hit
GTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGG2080.14085746984769787No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA2050.13882587172489452No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGA2050.13882587172489452TruSeq Adapter, Index 1 (96% over 29bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC1990.13476267547928786No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC1950.13205387798221674No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTG1850.12528188423953898No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC1790.12121868799393229No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT1750.11850989049686118No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA1730.11715549174832562No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT1720.11647829237405785TruSeq Adapter, Index 1 (95% over 24bp)
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC1700.11512389362552228No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACA1670.11309229550271896No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACAT1660.11241509612845119No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATC1650.1117378967541834No Hit
GCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT1650.1117378967541834No Hit
CATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCT1630.11038349800564784TruSeq Adapter, Index 1 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGG1630.11038349800564784RNA PCR Primer, Index 1 (96% over 29bp)
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC1630.11038349800564784No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC1620.10970629863138005No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCA1620.10970629863138005No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTC1590.10767470050857673No Hit
GATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC1570.10632030176004117No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC1550.10496590301150562No Hit
GTATAAGAGACAGCATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT1540.10428870363723783No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1540.10428870363723783TruSeq Adapter, Index 1 (96% over 29bp)
CATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTG1510.10225710551443451TruSeq Adapter, Index 1 (96% over 29bp)
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA1510.10225710551443451No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTC1500.10157990614016674No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCG1500.10157990614016674No Hit
CCAAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGA1480.10022550739163118TruSeq Adapter, Index 1 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)