FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002294371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2837091
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGC166590.5871859591391323No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC96460.3399961439375755No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC79690.28088630220179756No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC73740.259914116254995No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGC67520.23799025128203502No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC55240.19470647927754167No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG40750.1436330382070931TruSeq Adapter, Index 11 (96% over 28bp)
CTCCAGGATCGAACCCTGATCCTCTGTACCCGTTGTGACCATGAATGGACGTGGCGCTTTCATCGGGAGTTGATA33810.11917136249771333No Hit
CCTTAGAAGTGTGAGCAATCTTTAAGGCCCACTCTCCAGGATCGAACCCTGATCCTCTGTACCCGTTGTGACCAT30140.10623557721624016No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)