FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002294379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3207165
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGC2074966.469763794503869No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG938542.9263851407707433Illumina PCR Primer Index 7 (96% over 27bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC810742.5279023686028004No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC564771.7609633430147809No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC489901.527517293310447No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG463421.4449521618002192Illumina PCR Primer Index 7 (96% over 27bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCA452841.411963525418867No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG443771.3836830970654768No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGA443091.381562844443613No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG403631.2585258320042778No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG348141.085506982023064Illumina PCR Primer Index 7 (96% over 27bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGA338881.056634130142977No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG337401.0520194626718613No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGC334911.0442555964535658No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGT273320.8522168332468083No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG257690.8034822031295552Illumina PCR Primer Index 7 (96% over 27bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG239080.7454558776988399No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC191370.5966952121265977No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT189210.5899602920336185No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACT182200.5681029819170513No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT179670.5602143949562932No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG163510.5098272150014109Illumina PCR Primer Index 7 (96% over 27bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC159300.49670035685722436No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT142920.4456272128187979No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA139290.4343088054403188No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC139160.4339034630273154No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCT139110.43374756209923715No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG131150.40892813434918374RNA PCR Primer, Index 41 (96% over 31bp)
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC122170.3809283276663346No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC121120.3776544081766919No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATC119150.37151191161040986No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC118730.37020234381455275No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA110690.3451334745795742Illumina PCR Primer Index 7 (96% over 27bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT107080.33387742757232636No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGC106980.3335656257161699No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC104020.324336290773939No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC97270.30328966548337866No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA94780.29552579926508304No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT93330.2910046723508145No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC91680.28585994172423307No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCG82790.2581407567119247No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC78750.24554396172320414No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGA76450.23837251903160578No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG76100.2372812125350582No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC72380.2256821834860383No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT71330.22240826399639557Illumina PCR Primer Index 7 (95% over 22bp)
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG70890.22103633582930718No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACT70110.21860428135128687No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG69960.21813657856705218No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG68700.21420787517948095No Hit
ATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC67570.21068451420491308No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG66690.20794065787073632Illumina PCR Primer Index 7 (96% over 27bp)
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCA64700.20173580093322294No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTC63400.19768237680318912No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACAT63200.19705877309087622No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTC63040.1965598901210259No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC59980.18701875332263854No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT58760.18321477067752984No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG58710.1830588697494516Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG57440.1790989861762647Illumina PCR Primer Index 7 (96% over 27bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG56000.17460903944761183No Hit
GATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAC55990.1745778592619962No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG55600.17336183202298602No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATG55460.17292530942436699No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCT55150.171958723670282No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGA54540.17005673234772767No Hit
GGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG54140.1688095249231019Illumina PCR Primer Index 7 (95% over 23bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACAC52510.16372715466775173No Hit
GATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAC51830.1616069020458879No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG49260.15359359434266712TruSeq Adapter, Index 7 (96% over 26bp)
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG48820.15222166617557875No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA48820.15222166617557875No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCAC48760.15203458506188489No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG48040.14978961169755844No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT47930.14944662965578634No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCAT47380.14773171944692587No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGA47130.14695221480653473No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAC46520.14505022348398042No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT45870.14302351141896347No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC43980.13713045633760657No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGAC43060.13426187926096725No Hit
GTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC42740.13326411332126661Illumina PCR Primer Index 7 (95% over 21bp)
GTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACG42720.1332017529500353No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCA42660.13301467183634144No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGC42350.13204808608225643No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG41640.12983429290354565No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTC41630.12980311271793002No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCC39830.12419067930711392No Hit
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT39300.12253812946948472No Hit
AGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTC38840.12110384093116505No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA38650.12051141740446782No Hit
GCGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGA37930.11826644404014136No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG36960.1152419660354238No Hit
GTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGA36640.11424420009572317Illumina PCR Primer Index 7 (96% over 27bp)
GCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT36050.11240456914440011No Hit
GTCCAGGGTGGACATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT35930.11203040691701238No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGG35680.11125090227662125Illumina PCR Primer Index 7 (96% over 27bp)
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACG35030.10922419021160432No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGG34490.10754046018835951No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA34080.10626207257811807Illumina PCR Primer Index 7 (96% over 27bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCG34040.10613735183565548No Hit
TCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT33920.10576318960826774No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGG33830.10548256793772694Illumina PCR Primer Index 7 (96% over 27bp)
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC33260.10370529735763516No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATGTCGTATGCCGTC33050.10305051345970663No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGC33020.1029569729028597No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGG32430.10111734195153664Illumina PCR Primer Index 7 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)