FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002294397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500782
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC50682620.266700576059808No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC2291259.162134084458382No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC2095818.3806185425199RNA PCR Primer, Index 32 (95% over 24bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC1891427.563314195319704RNA PCR Primer, Index 32 (95% over 24bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC1550926.2017400956980655RNA PCR Primer, Index 32 (95% over 24bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC1035804.141904412299833RNA PCR Primer, Index 32 (95% over 24bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTC792513.169048721559896No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAA640262.5602391571916305No Hit
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC270591.082021543661143RNA PCR Primer, Index 32 (95% over 24bp)
GGTCGGTAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGC261521.0457528884964782RNA PCR Primer, Index 32 (96% over 30bp)
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTT171610.6862253487109232RNA PCR Primer, Index 32 (96% over 26bp)
GTCCAGGGTGGACATGGGCACACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC169750.6787876752151927No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGC163640.654355317656637No Hit
CTAGTTTCCTCTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC129910.5194775074356741RNA PCR Primer, Index 32 (95% over 24bp)
GTCCAGGGTGGGCATGGGCACACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC118900.4754512788399789No Hit
CTGCTTCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTG118890.4754112913480663RNA PCR Primer, Index 32 (96% over 33bp)
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATC101230.4047933806305388No Hit
GCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC92170.3685647129577868RNA PCR Primer, Index 32 (95% over 24bp)
CATCCGCAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT88010.3519299163221744RNA PCR Primer, Index 32 (95% over 23bp)
TCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC87180.34861095449343443RNA PCR Primer, Index 32 (95% over 24bp)
CCCGATGAGCAGCACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC85670.34257284321464243RNA PCR Primer, Index 32 (95% over 24bp)
GCTCTCAGCTCCGTAGTGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG83770.33497521975126177RNA PCR Primer, Index 32 (95% over 22bp)
CATCCATAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT83580.33421545740492375RNA PCR Primer, Index 32 (95% over 23bp)
GTTTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTAT75510.3019455514315122No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGC72570.2901892288092285No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTC72570.2901892288092285No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTC71170.2845909799414743No Hit
CCCGATGAGCAGCCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT68870.27539385680159245RNA PCR Primer, Index 32 (95% over 23bp)
GTTTCCACGTCAGAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG67520.2699955453934009RNA PCR Primer, Index 32 (95% over 22bp)
GGTCATTGTCACTGGTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT64590.2582792102630297RNA PCR Primer, Index 32 (95% over 23bp)
GTTTCCAGGTCAAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATC62660.25056162432391144No Hit
CATCCGCAGCGTCCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC59220.23680592710600126RNA PCR Primer, Index 32 (95% over 24bp)
AAAGTTGAAATGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTC56450.2257293918462305No Hit
CAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTG56210.2247696920403298RNA PCR Primer, Index 32 (96% over 33bp)
CATCCATAGCGTCCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC54890.21949134310787582RNA PCR Primer, Index 32 (95% over 24bp)
CTTCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC54290.21709209359312406RNA PCR Primer, Index 32 (95% over 24bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTC52640.21049415742755667No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTC49830.1992576722001358No Hit
GCTCTCAGCTCCGTAAAGGTGGAGCTCATCTTCATTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCA49690.19869784731336038No Hit
GTTTCCAGGTCAGAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG47690.1907003489308544RNA PCR Primer, Index 32 (95% over 22bp)
GTTTCCAGGTCAAGGGTAACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAA46240.18490216260353762No Hit
CTTCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC43670.17462537718201748RNA PCR Primer, Index 32 (95% over 24bp)
CTAGTTTCCTTTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC42060.16818739098410018RNA PCR Primer, Index 32 (95% over 24bp)
AAAGTTGAAATGTCAGGGTCATGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTC37910.1515925818404003No Hit
CATCCGCAGCGTCATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC36280.14507462065865798RNA PCR Primer, Index 32 (95% over 24bp)
CATCCGTAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC33530.13407806038271228RNA PCR Primer, Index 32 (95% over 24bp)
AAAGTTGAAATATCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTC33090.132318610738561No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAAGCTCGTATGC32450.12975941125615906No Hit
GTCCAGGGTGGAGCTCATCTTCATTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGT32230.12887968643408343No Hit
GGCTGTGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC31960.12780002415244512No Hit
CATCCGCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTG28700.1147641017889604RNA PCR Primer, Index 32 (96% over 33bp)
GGCTGTGGTGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGC27990.1119249898631708RNA PCR Primer, Index 32 (96% over 30bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATGTCGTATGC27910.11160508992787058No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATG27840.11132517748448285No Hit
GACCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC26380.1054870036652535No Hit
GTTTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTAT25490.10192811688503836No Hit
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTCTT25440.10172817942547571No Hit
GTCCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTAT25310.10120834203061282No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)