FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002294447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182821
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG208841.765609504734867No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCG132951.1240077746336934No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG90690.7667263262995838No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACATC70230.5937500264198894No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCT69750.589691931408049No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCC69460.5872401656717289No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTGACGCT62850.5313568156128442No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAAT59380.5020201704230818No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT55000.4649900534400387No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCC50280.42508545249027535No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATC47750.4036959100320336No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAAT45610.3856035697709121No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCT40080.3388509334886682No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGCTGTCTCTTATACACATCTGACGCTGC38990.3296356760659474No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTGACGCTGCCGACG38880.32870569595906735No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACAT38000.3212658551040267No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACG37430.31644686727746635No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATC34990.2958182176339446No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGCTGTCTCTTATACAC34700.29336645189762445No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGCTG32340.2734141514227427No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGACTGTCTCTTA30480.25768903325186143No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTGACGC29790.251855521672341No Hit
CAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGC28690.2425557206035402No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATC28120.2377367327769798No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGC28050.2371449272544197No Hit
TATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCGA27200.22995871733761916No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAA26860.22708423337089886No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACAT26650.22530881680321874No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAG26620.22505518586497872No Hit
GTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGACGG26430.22344885658945857No Hit
GTATCAACGCAGAGTACATGGGGGTGTGGGTCTTGTCGCAGCTCTTGGGCTCGCTTCCGCTCTGGAAGTACAGGT25540.21592447208833798No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGACGG24950.21093639696961752No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGCCGA23390.19774758818113647No Hit
CCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACAC22710.19199862024769598No Hit
CACAAAATCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTG21880.18498149762305538No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAATTA21800.18430514845441534No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGC21450.18134612084161508No Hit
ATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCGAC21130.17864072416705487No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGC21080.17821800593665482No Hit
ACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCGACGAAT20940.17703439489153472No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTGACGCTGCC20790.17576624020033463Clontech Universal Primer Mix Short (95% over 21bp)
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGACGAA20540.17365264904833444No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTCTGTCTCTTATACACATCTGACGCTGCCGACGAAT20250.17120088331201425No Hit
GTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACATCTGACGCTGCCGAC20230.17103179601985422No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACAT19790.16731187559233393Clontech Universal Primer Mix Short (95% over 21bp)
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTGACGCTGCCGAC19780.16722733194625392No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGCTGTCTCTTATACACATCTGACGCTG19750.1669737010080139No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGTAA18980.16046384025985336No Hit
GAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTGACGCTGCCGA18470.156152114309773No Hit
GTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCG18220.1540385231577728No Hit
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGA18200.1538694358656128No Hit
TCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTA17890.15124858283713258No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCCTGTCTCTTATACACATCTGACGCTGCCGA17770.1502340590841725No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAATTA17480.14778229334785228No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATCTGTCTCTTATACACATCTG17430.14735957511745226No Hit
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTG17390.14702140053313223No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTG17120.14473872208897204No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGAC16950.14330148010561192No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACCTGTCTCTTATACACATCTGACGCTG16800.14203332541441183No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGCTGTCTCTTATACA16750.14161060718401178No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGCT15690.13264898069953104No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGCTGTCTCTTATACACATCTGACGCTGCCGA15450.13061993319361087Clontech Universal Primer Mix Short (95% over 22bp)
TATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACATCT15260.12901360391809072No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATCTGTCTCTT15160.12816816745729068No Hit
GTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTG15080.1274918182886506No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCTGTCTCTTATACA15030.12706910005825056No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTCTGTCTC14870.12571640172097046No Hit
TATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAATT14710.12436370338369035No Hit
ACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTGACGCT14670.12402552879937032No Hit
TATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTGACGCTG14330.12115104483265007No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGACTGTCTCTT14200.12005197743361No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTCTGTCTCTTATACACATCTGACGCT14140.11954471555712995No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGTCT14120.11937562826496992No Hit
GAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGAC14090.11912199732672991No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGCCGACGAATT14060.1188683663884899No Hit
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCA14000.11836110451200985No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGCTGCCGACGAATTA13700.11582479512960964No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGCTGTC13700.11582479512960964No Hit
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACAT13630.11523298960704959Clontech Universal Primer Mix Short (95% over 21bp)
CCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATA13560.11464118408448953No Hit
GGTATCAACGCAGAGTACATGGGGGTGTGGGTCTTGTCGCAGCTCTTGGGCTCGCTTCCGCTCTGGAAGTACAGG13550.11455664043840952No Hit
CAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTG13420.11345757303936944No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCT13380.1131193984550494No Hit
GACAGTCGCTACGGGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACC13340.11278122387072939No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACG13300.11244304928640936No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACGAATTA12960.10956856531968912No Hit
CACAAAATCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCT12940.1093994780275291No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAATT12930.10931493438144911No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGAC12700.10737043052160894No Hit
GTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAG12440.10517229572352875No Hit
ATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTG12420.10500320843136873No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAG12330.10424231561664866No Hit
GGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACA12120.10246689904896854No Hit
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCC11910.10069148248128838Clontech Universal Primer Mix Short (95% over 21bp)
CCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCG11840.10009967695872833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)