FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002294455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282338
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG512132.2438832460398066No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCG214030.9377664482648932No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG157810.6914400934480344No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAAT150220.658184721106164No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTGACGCT140760.6167359961583254No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAATTA134390.5888260196342522No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAAT126450.5540371320987514No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCT123940.5430396374244305No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAG122250.5356349497751867No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAATTA122140.5351529878571886No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCC121410.5319545133104737No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACATC112300.4920393035562656No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT102040.4470854010229861No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCT100400.4398997869728322No Hit
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGA93500.40966763029840453No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCC92950.40725782070841393No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGC85860.376193184357444No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGCTGTCTCTTATACACATCTGACGCTGC83360.3652395044029412No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAA80160.3512187940611776No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATC79610.348808984471187No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTGACGC73860.3236155205758306No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGC73560.32230107898129023No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAATT70840.3103834751907912No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTGACGCTGCCGACG70210.30762314784225653No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACG69510.3045561174549957No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACAT69060.3025844550631852No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGCTGTCTCTTATACAC67800.2970638003661158No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGACGAA67590.29614369124993756No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGACGAATT64350.28194772202890195No Hit
CAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGC62370.2732724075049357No Hit
CCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATA59530.26082902707662053No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTGACGCTGCC58930.25820014388753987Clontech Universal Primer Mix Short (95% over 21bp)
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATC57190.2505763826392059No Hit
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCA56760.24869234968703147No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGC56300.24667687257540297No Hit
CCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACAC55960.24518717210159058No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTGTCTCTTATACACATCTGACGCTGCCGACG54640.23940362908561308No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGCTGTCTCTTATACACATCTGACGCTGCCGA51100.2238932182700371Clontech Universal Primer Mix Short (95% over 22bp)
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGCTG49890.2185916371720578No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACAT49070.21499883014698085No Hit
ACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCGACGAAT48120.21083643176426978No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATC47840.20960961960936547No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGACTGTCTCTTA46830.20518433290774635No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACCTGTCTCTTATACACATCTGACGCTG45820.20075904620612722No Hit
GTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTG45740.20040852844758314No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGA45730.20036471372776513No Hit
CCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACG45190.19799871885759251No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACAT45080.1975167569395944Clontech Universal Primer Mix Short (95% over 21bp)
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTCTGTCTCTTATACACATCTGACGCTGCCGACGAAT44030.19291621135870324No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGCTGTCTCTTATACACATCTGACGCTG43940.19252187888034114No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACGAATTA43260.18954247793271636No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCCTGTCTCTTATACACATCTGACGCTGCCGAC42620.18673833586436364No Hit
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCC42420.18586204146800342Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCG42160.18472285875273514No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCCTGTCTCTTATACACATCTGACGCTGCCG41380.18130531060693025No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGCTGTCTCTTATACA41310.18099860756820418No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGCTGCCGACGAATTA41000.17964035125384584No Hit
CTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGA39160.17157844280733178No Hit
ATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCGAC39140.17149081336769575No Hit
CACAAAATCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTG38560.16894955961825112No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGCCGACGAATTAG38460.168511412420071No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCTGTCTCTTATACACATCTGACGCTGCCGA37240.16316601660227364No Hit
CCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGA37090.16250879580500346No Hit
GTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGACGG37070.16242116636536746No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGACGG36410.15952939485737871No Hit
GCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGAC36060.1579958796637483No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACG35770.156725252789026No Hit
CCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCG35770.156725252789026No Hit
TATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCGA35770.156725252789026No Hit
GAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG35650.15619947615120985No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTGACGCTGCCGAC35540.15571751423321173No Hit
GTATCAACGCAGAGTACATGGGGGTGTGGGTCTTGTCGCAGCTCTTGGGCTCGCTTCCGCTCTGGAAGTACAGGT35410.1551479228755776No Hit
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAG35080.15370203712158323No Hit
CAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTG34220.14993397121723426No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTG34100.14940819457941812No Hit
GAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTGACGCTGCCGA32850.14393135460216674No Hit
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTG32400.14195969221035623No Hit
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTGACGCTGC32280.1414339155725401Clontech Universal Primer Mix Short (95% over 21bp)
GTTAGAGCCTTCTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACAT32150.14086432421490594Clontech Universal Primer Mix Short (95% over 21bp)
CCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACA32100.1406452506158159No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCCTGTCTCTTATACACATCTGACGCTGCCGA31620.13854214406455134No Hit
GTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACATCTGACGCTGCCGAC31580.1383668851852793No Hit
TCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTA31310.137183887750193No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGCCGA30720.13459881928093034No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACG30640.13424830152238626No Hit
AGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGA30420.13328437768639No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGCCGACGAATT29770.13043642089821927No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGCT29560.12951631178204107No Hit
TATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAATT29200.12793898186859265No Hit
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGCTGTCTCTTATACACATCTGACGCTGCCG27840.12198017997334314Clontech Universal Primer Mix Short (95% over 22bp)
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGCT27630.12106007085716489No Hit
GTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAG27550.12070955309862079No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCT27550.12070955309862079No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTGACGCTGCCGACGAATTA27340.11978944398244257No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGACTGTCTCTT27300.11961418510317051No Hit
ACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGACG27060.11856263182753826No Hit
GGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTC27030.11843118766808422No Hit
ACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACATCTGACGC26810.11746726383208797No Hit
TATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAGG26780.11733581967263394No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGAC26380.1155832308799135No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGACG26210.1148383806430073No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGCTGTCTCTTATACACATCTGACGCTGCCGACGAAT26040.11409353040610112No Hit
ACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTGACGCT25870.11334868016919493No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTCTGTCTCTTATACACATCTGACGCT25270.11071979698011425No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTCTGTCTCTTATACACATCTGACGC25050.109755873144118No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAG24980.10944917010539193No Hit
CTCCCTATAGTGAGTCGTATTACTGCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGC24760.10848524626939568Clontech Universal Primer Mix Short (95% over 21bp)
GGTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGAAGGCTCTAACCTGTCTCTTATACACA24710.10826617267030562Clontech Universal Primer Mix Short (95% over 21bp)
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCTGTCTCTTATACA24140.10576873364067899No Hit
CCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTC23980.1050676981235908No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATCTGTCTCTTATACACATCTGACGCTGCCGACGAA23930.10484862452450076No Hit
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACCTGTCTCTTATACACATCTGACGCT23860.10454192148577468No Hit
ATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAATTA23790.10423521844704861No Hit
GTATCAACGCAGAGTACATGGGGCTGTCTCTTATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTC23550.10318366517141633No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTGTCTCTTATACACATCTGACGCTGCCGAC23190.10160633525796792No Hit
ACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTGACGCTGCCGAC22910.10037952310306361No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)