FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG936.365503080082135No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG795.4072553045859No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG745.065023956194388No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCGGGTAGCCGGCAA594.038329911019849No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC573.9014373716632447No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA563.8329911019849416No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT553.764544832306639No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT553.764544832306639No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT513.4907597535934287No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT483.285420944558522No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATG473.2169746748802193No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT412.8062970568104038No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTGTT241.642710472279261No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCTGGAGGACCGCATTGACTACCTGA241.642710472279261No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC161.0951403148528405No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACCCTTTCAACAACTCCTGTT161.0951403148528405No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATTATTCTTTTATTGGCATGTGCCAATA161.0951403148528405No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACTACCTGA151.0266940451745379No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCGCTTGGCCTTGTCGCGGCTCTTG130.8898015058179329No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGC120.8213552361396305No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCNCCTGCGCACATG100.6844626967830253No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTNGGAAGAAGTAGG90.6160164271047228No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTTCCCCTGCGCACATG80.5475701574264202No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGNCCCCCTCAGTGA80.5475701574264202No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC70.4791238877481177No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGT70.4791238877481177No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGTGCCATTCAGGTA60.41067761806981523No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATT50.34223134839151265No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATAACAGTTGCTTTGTTGACAACAGTCA50.34223134839151265No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC50.34223134839151265No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGTCCTGCTTGAGGATGGGTAGGGGGAT40.2737850787132101No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCNATTGGGATCATG40.2737850787132101No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGTCCTGCTTGGGGATGGGTAGGGGGAT40.2737850787132101No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCNTGGCAGTATTAT40.2737850787132101No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGNGAAGATGCCGAT40.2737850787132101No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCANAGCATCATCCAT30.20533880903490762No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCACGC30.20533880903490762No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACNCTTAACGGCCCC30.20533880903490762No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCCGGTCGCGAAGGGGCCCTG30.20533880903490762No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCNGGTAGCCGGCAA30.20533880903490762No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGNGAAGGGGCCCTG30.20533880903490762No Hit
CAGTGACATGTATTCCCCCTCTTACCTTACGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT30.20533880903490762No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCCCTCTTAACGGCCCC30.20533880903490762No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAAACCACTGCTGCAGAACTTGATAGCCACAC30.20533880903490762No Hit
CAAGCAATCAAGACCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT20.13689253935660506No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCNTTGACTACCTGA20.13689253935660506No Hit
CTTCTTGTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCCC20.13689253935660506No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTNGTCGCGGCTCTT20.13689253935660506No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATACGCAGCACTCTGCACAGCATCATCCAT20.13689253935660506No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACCAAAGCCACCGCAGCAATAAGCTGGAGGACCGCATTGACTACCTGA20.13689253935660506No Hit
CTTTGACAGCACCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG20.13689253935660506No Hit
CAAGCAGTCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT20.13689253935660506No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCACTGCTGCAGAACTTGATAGCCACACC20.13689253935660506No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGC20.13689253935660506No Hit
AAAGTCTCTGACGTGGATGCGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGTGCCATTCAGGTA20.13689253935660506No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCTGGAGGACCTCATTGACTACCTGA20.13689253935660506No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTTTCAGAATGGCCGGGATGCTGGTGGC20.13689253935660506No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCATCGGGTAGCCGGCAA20.13689253935660506No Hit
CCTTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT20.13689253935660506No Hit
ACCTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGT20.13689253935660506No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAGGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT20.13689253935660506No Hit
GTCGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT20.13689253935660506No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCTGGAGGACCGCNTTGACTACCTGA20.13689253935660506No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCACC20.13689253935660506No Hit
CTTTGACAGCATCACGAGTTTCACCGGTATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG20.13689253935660506No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTCGG20.13689253935660506No Hit
CTTTTACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG20.13689253935660506No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTTCGGGGGGCCCCCTCAGTGA20.13689253935660506No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGCTCATG20.13689253935660506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)