FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT1217.91044776119403No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCT45.970149253731343Illumina PCR Primer Index 6 (95% over 22bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAGCTCGTATGCCGTCTT22.9850746268656714No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATGTCGTATGCCGTCTT11.4925373134328357No Hit
CAAGCAATCAAGGCCAGTCGCTTATACACATCTCAGAGCCCACGATACCCACGTATATCTCGTATGCCGTCGTAT11.4925373134328357No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAACTCGTATGCCGTCTT11.4925373134328357No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCC11.4925373134328357Illumina PCR Primer Index 6 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCTGTCTT11.4925373134328357No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGGCCCACGTATATCTCGTATGCCGTCTT11.4925373134328357No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCCCGTATGCCGTCTT11.4925373134328357No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATATCCGAGCCCACGAAACCCAAGTAAAGCTCGTATGCCGTCTT11.4925373134328357No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACGCATCTCCGAGCCCACGAGGCCCACGTATATCTCGTATGCCGTCTT11.4925373134328357RNA PCR Primer, Index 37 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGACCACGAGACAAACGTCTATCTCGTATGCCGTCTT11.4925373134328357No Hit
CAAGCAACCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGNATGCCGTCTTCT11.4925373134328357TruSeq Adapter, Index 6 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAGCTCGTATGCCGTCTTCT11.4925373134328357Illumina Single End Adapter 1 (95% over 21bp)
GCACAGGTTATACGCATTGGCGGACGTACTCAGAGGGACTCAGCACCGCCTAGCAGGTCGCGAAGGGGCCCTGGG11.4925373134328357No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACATATATCTCGTATGCCTTCTT11.4925373134328357No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCACGTATGCCGTCTT11.4925373134328357No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCCT11.4925373134328357No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACTTATAGCTCGTATGCCGTCTTCT11.4925373134328357TruSeq Adapter, Index 6 (95% over 21bp)
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT11.4925373134328357No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAACTCGTATGCCGTCTTCT11.4925373134328357TruSeq Adapter, Index 6 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCTTATGCCGTCTT11.4925373134328357No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGATACCCACGTATAGCGCGTATGCCGTCTT11.4925373134328357No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACGTATATCTCGTATGCCGTCTT11.4925373134328357No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCCGCACCCTTTCNACAACTCCTGTT11.4925373134328357No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT11.4925373134328357No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAACGTAGAGCTCGTATGCCGGCTT11.4925373134328357No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATCTCTCGTATGCCGTCTTCT11.4925373134328357TruSeq Adapter, Index 6 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAGATCGTATGCCGTCTT11.4925373134328357No Hit
CAAGCAAAAGAACAGCCTGTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCT11.4925373134328357RNA PCR Primer, Index 37 (95% over 23bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAGCGCGTATGCCGTCTT11.4925373134328357No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCNACAACTCCTGTT11.4925373134328357No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCGCGGAGAGCTCGTATGCCGGCGT11.4925373134328357No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA11.4925373134328357No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGC11.4925373134328357No Hit
CAACCAAAAGAACAGCCTGTCTCTGATACACATCTCCGAGCCCACGAGACCCACGTATAACTCGTAGGCCGTCTT11.4925373134328357No Hit
CAACCAAAAGAACAGCCTGTCTCTTATCCACATCTCCGAGCCCACTAGACCCACGTATATCTCGTATGCCGTCTT11.4925373134328357No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGCGCCCACTATACCCACGTATATCTCGTATGCCGTCTT11.4925373134328357RNA PCR Primer, Index 37 (95% over 21bp)
GTAAAGGTTGAAATGTCAGGGCCAGTCACAGTTTGGACTAGACAAACCACTGATGCAGAACTTGATAGGCACACC11.4925373134328357No Hit
CAAGCAATCAAGGCCAGTCTATTATACAGATCTCCGAACCCACGAAAAAGAAGTATAACTCGTAGGACGTCTTAT11.4925373134328357No Hit
GCACAGGTCGTATACGCAGTGGCGGAAGTACTCAGAGGGACTCAGCACCACCTGGCAGGTCGCGAAGGGGACCTG11.4925373134328357No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACACAAGCCACCCCAGCAATACGCTGGAGGACCGCNTTGACTACCTGA11.4925373134328357No Hit
CAACCAAAATAACAGCCTGTCTCTTATACACATCTCCTAGCCCACGATACCCACGTCTATCTCGTATGCCGTCTT11.4925373134328357No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACTAAACCCACTTATATCTCGTATGCCGTCTT11.4925373134328357TruSeq Adapter, Index 25 (95% over 24bp)
CAACCAAAAGAACAGCCTGACTCTTATACACATCTCCGAGCCCAAGAGACCCACGTAGATAGCGTATGCCGTCTT11.4925373134328357No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCAACGTATATCTAGTATGCCGTATTCT11.4925373134328357No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTNGTATGCCGTCTT11.4925373134328357No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAACTCGTATGCCGTCTT11.4925373134328357No Hit
CAAGCAATCCAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCT11.4925373134328357Illumina PCR Primer Index 6 (95% over 22bp)
CCTTTAACACGCATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTCTATCTCGTATGCCGTCTT11.4925373134328357RNA PCR Primer, Index 16 (95% over 22bp)
AACTGTAGCTTACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGGCGTCTGCTTGAAAAAAAAA11.4925373134328357No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)