FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG713.461538461538462No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT59.615384615384617No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCT47.6923076923076925Illumina PCR Primer Index 6 (95% over 22bp)
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT23.8461538461538463No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACGTATATCTCGTATGCCGACTT11.9230769230769231No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCATTCTT11.9230769230769231No Hit
CAAGCAAAAGAACAACCTGTCTCTTATACACCTCTACGAGCCCACGAGACCCACGTAGAGCTCGTATGCCGTCTT11.9230769230769231No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAAGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG11.9230769230769231No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATATNGTATGCCGTCTT11.9230769230769231No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTC11.9230769230769231RNA PCR Primer, Index 37 (95% over 22bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCTGTCTT11.9230769230769231No Hit
CAACCAAAAGAACAGCCTGTCTCTTATAACATCTCCGAGCCCACGAGACCCACGTATCGCTCGTATGCCGTCTTC11.9230769230769231No Hit
GTAACAGTTGAAATGTCAGGGCCGCGGACACGGGGGGCACTGCGTCATGCTTGAGGATGGGTAGGGGGATCAGGT11.9230769230769231No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGCTTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT11.9230769230769231No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCACTCAGCACCCTTTCACCAACTCCTGTT11.9230769230769231No Hit
GAAGCAGTGGATCGCCGAGCCCACGAGACGCTCATGAAACTCGTATGCCGTCCTCTGCTTGAAAAAAAAAACAAT11.9230769230769231Illumina Single End Adapter 1 (95% over 22bp)
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCGGATCGAGAAGGGGCCCTG11.9230769230769231No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAAACCCACGTATATAACATATGCCGTCTA11.9230769230769231No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCGCACGAGACACACGGATATGGCGTATGCCGGCTT11.9230769230769231No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATATCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT11.9230769230769231No Hit
GCACAGGTCGTATACGCCTTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCCGGTCGCGAAGGGGCCCTG11.9230769230769231No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACACGTAGATCTCGTATGACGTATT11.9230769230769231No Hit
CAACCAAAAGAACAGCCTGTCTCTTATCCACATCTCCGCGCCCACTAGACCCACGTCTAGCTCGTATGCCATCTT11.9230769230769231No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTAGTATGCCGTCTTCT11.9230769230769231TruSeq Adapter, Index 6 (95% over 21bp)
ACACAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACTACCTGAA11.9230769230769231No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGNATACCGTCTTCT11.9230769230769231No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT11.9230769230769231No Hit
CAAGCAATCAAGGCCAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATCTATCGTATGCCGTC11.9230769230769231No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAACCCACGAGACCCACGTATATCTCGTATGCCGTCTT11.9230769230769231RNA PCR Primer, Index 37 (95% over 21bp)
CAAGCAATCAAGTCCATTCTCTTATACACATCTCCGATCCCACGGGACCCACGTATATCTCTTATGCCGTCTTCT11.9230769230769231TruSeq Adapter, Index 6 (95% over 21bp)
CAAACAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACATATATATCATATGCAGTCTT11.9230769230769231No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAACGTGTATCTCGTATTCCGTCTT11.9230769230769231No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGATACCCACGTATATCTCGTATGCCGTCTT11.9230769230769231RNA PCR Primer, Index 37 (95% over 21bp)
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT11.9230769230769231No Hit
CAACCAAAAGAACAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCACGTATATCTCGTATGCCGTCTT11.9230769230769231No Hit
CAACCAAAAGAACAGCCTGTCTCTTATCCACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTT11.9230769230769231No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATAACTCGTATGCCGTCTTCT11.9230769230769231TruSeq Adapter, Index 6 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCAACGAGACCCACGTATATCTAGCATGTCGTCTTCT11.9230769230769231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)