FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5885
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATTATTCTTTTATTGGCATGTGCCAATA2353.993203058623619No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG2313.925233644859813No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG2153.653355989804588No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG2083.5344095157179267No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCGGGTAGCCGGCAA1813.0756159728122343No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGTGCCATTCAGGTA1732.9396771452846218No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT1422.4129141886151233No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT1332.259983007646559No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGC1282.1750212404418012No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTTTCAGAATGGCCGGGATGCTGGTGGC1262.141036533559898No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTGGAAGGCGGGGACCCAACCTTCCCAC1252.1240441801189465No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC1242.1070518266779947No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT1242.1070518266779947No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATG1151.954120645709431No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGT1121.9031435853865761No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT1081.8351741716227696No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATAACAGTTGCTTTGTTGACAACAGTCA1051.7841971112999149No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCTGGAGGACCGCATTGACTACCTGA1051.7841971112999149No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT1011.7162276975361088No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACTACCTGA971.6482582837723025No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA931.5802888700084963No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACCCTTTCAACAACTCCTGTT741.2574341546304162No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGACACCGGGTCATTCTTTTTAATATGTGCTTTAGACTCTCCACT721.2234494477485132No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTGTT721.2234494477485132No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAAACCACTGCTGCAGAACTTGATAGCCACAC570.9685641461342397No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCACTGCTGCAGAACTTGATAGCCACACC550.9345794392523363No Hit
CTGTTTTCATCCCACAGGTTGTTATTTTGTACTTTGAGCCAAGCACATTTCGCTGTATTCTCCTAAGATGGGCTC540.9175870858113848No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGGCACCGGGTCATTCTTTTTAATATGTGCTTTAGACTCTCCACT540.9175870858113848No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCTCCCCCTGCACCAGGGGAAGCAGTGG360.6117247238742566No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC260.44180118946474084No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGTCCTGCTTGAGGATGGGTAGGGGGAT260.44180118946474084No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC230.3908241291418862No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATTATTCTTTTATTGNCATGTGCCAATA220.3738317757009346No Hit
AGCCAAAGGGTCTAAAGAAGTGGAAGTCACTCTTGTTCGGAAAGACAAAGGGCGCCAGAAAACAACCCGATCACG210.356839422259983No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTNGGAAGAAGTAGG190.32285471537807986No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCNGGTAGCCGGCAA160.27187765505522515No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTGGAAGGCGGGGACNCAACCTTCCCAC160.27187765505522515No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCACGC160.27187765505522515No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCNCCTGCGCACATG150.2548853016142736No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGNGCCATTCAGGTA130.22090059473237042No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGTCCTGCTTGGGGATGGGTAGGGGGAT130.22090059473237042No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACNCTTAACGGCCCC120.20390824129141888No Hit
AGCCAAAGGGTCTAAAGAAGTGGAAGTCGCTCTTGTTCGGAAAGACAAAGGGCGCCAGAAAACAACCCGATCACG120.20390824129141888No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTTTCAGAATGGCCGNGATGCTGGTGGC100.16992353440951571No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCANAGCATCATCCAT100.16992353440951571No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCTGGAGGACCGCNTTGACTACCTGA90.15293118096856415No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGTGCCATTCAGTTA90.15293118096856415No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCNACAACTCCTGTT90.15293118096856415No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC90.15293118096856415No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGNTATCAGCCAAGT80.13593882752761258No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGNGAAGATGCCGAT80.13593882752761258No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCNTTGACTACCTGA80.13593882752761258No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCNATTGGGATCATG80.13593882752761258No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGNGAAGGGGCCCTG80.13593882752761258No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTTCCCCTGCGCACATG70.11894647408666101No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACCTG70.11894647408666101No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCNTGGCAGTATTAT70.11894647408666101No Hit
CCTTTAACAAGTATGCCTGTCAGCATCACACCGGTGACCAGTCCTGAGGCTAGCACCCTTTCAACAACTCCTGTT70.11894647408666101No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCANAGCATCATCCAT70.11894647408666101No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATTATTCTTTTATTGGCATGTGCCACTA70.11894647408666101No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGNTATCAGCCAAGC60.10195412064570944No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCTCATTGACTACCTGA60.10195412064570944No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGAGCACCAGCAGCGGGTAGCCGGCAA60.10195412064570944No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAATCAGCACCCTTTCNACAACTCCTGTT60.10195412064570944No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATAACAGTTGCTTTGNTGACAACAGTCA60.10195412064570944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)