FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG1110.476190476190476No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT109.523809523809524No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT87.6190476190476195No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG76.666666666666667No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTGCTTGGCCTTGTCGCGGCTCTT43.8095238095238098No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATG32.857142857142857No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC32.857142857142857No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC32.857142857142857No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTTCCCCTGCGCACATG21.9047619047619049No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT21.9047619047619049No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCGGGTAGCCGGCAA21.9047619047619049No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTGTT21.9047619047619049No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC21.9047619047619049No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT21.9047619047619049No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGCGCTACTTGGCCTTGTCGCGGCTCTT10.9523809523809524No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCACTG10.9523809523809524No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGGGCGCCATGTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC10.9523809523809524No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGTGGCCCCCTCAGTGA10.9523809523809524No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCGGGTGGCCGTCCA10.9523809523809524No Hit
GTTTCCAGGTCAAGGGTAACGTAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA10.9523809523809524No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACATGTGCACCACTTCTGCCCCTGCGCACATG10.9523809523809524No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTGCCCATAAGCAGCACTCTGCACAGCATCATCCAT10.9523809523809524No Hit
GGCTGTGGTGTGAGTCCTGGGGGCGGCCGTAGCTCGCCATTTGCACCCTCAGCTCGCGGCCGTCCAGCACGGCCC10.9523809523809524No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCANAGCATCATCCAT10.9523809523809524No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC10.9523809523809524No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTNGGAAGAAGTAGG10.9523809523809524No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGTGCCCGCGCCCC10.9523809523809524No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAGGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT10.9523809523809524No Hit
CAGTGCCATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTAT10.9523809523809524No Hit
GGCTGTGGTGTGAGTCCAGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC10.9523809523809524No Hit
TCTCTTGCTCGCATATGCCTCCCAGCGGCTCCGGGGCGGCAGGTGGGTCGGGCGGCTGCGCGGGGCCCGCGCCCC10.9523809523809524No Hit
CAAGCAATCAAGGCCAGGCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT10.9523809523809524No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCCGACCAGT10.9523809523809524No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGAGACTCAGCACCGCCTGGCAGGTCGCGAGGGGGCCCTG10.9523809523809524No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGTTCGGTAGTACCTGTGCACCACTTCTGCNCCTGCGCACATG10.9523809523809524No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGTAGGTCGCGAAGGGGCACTG10.9523809523809524No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTGGCGCTGCTTGGCCTTGTCGCGGCTCTT10.9523809523809524No Hit
CAGTGAATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCNGGCAGTATTATT10.9523809523809524No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGGCACCGGGTCATTCTTTTTAATATGTGCTTTAGACTCTCCACT10.9523809523809524No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATACGCAGCACTCTGCACAGCATCATCCAT10.9523809523809524No Hit
ACACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG10.9523809523809524No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA10.9523809523809524No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCAAAGCAATAAGCTGGAGGACCGCATTGACTACCTGA10.9523809523809524No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTTGGGCGGGCGGCTGCCCGGGTCACGCTCACC10.9523809523809524No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATTATTCTTTTATTGGCATGTGCCAATA10.9523809523809524No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGNGAAGGGGCCCTG10.9523809523809524No Hit
TAAAATAGTAGCCCTCCCGCACTCACCGGGTCCGGTCGGTGGTGCCTGTGCACCACTTCTGCCCCTGCGCACATG10.9523809523809524No Hit
ATAGCAGAATCATCGTGGGCTCCAGAAGTGCCCACGTTGCTCTTGCTGCTCCCCCTGCACCAGGGGAAGCAGTGG10.9523809523809524No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTATCGCTCTGTCCACTCTTAACGTCCCC10.9523809523809524No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCACGCGCCCC10.9523809523809524No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCGCC10.9523809523809524No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCNTGGCAGTATTAT10.9523809523809524No Hit
CAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCTCCCCCTGCACCAGGGGAAGCAGTGGCGGC10.9523809523809524No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCANAGCATCATCCAT10.9523809523809524No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTGTGATGGTCGACATTATTCTTTTATTGGCATGTGCCAATA10.9523809523809524No Hit
CACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTGG10.9523809523809524No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGNGACGGGGCCCTT10.9523809523809524No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAACACCGCGTGGCGGGTCGCGAAGGGGCCCTG10.9523809523809524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)