FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002294508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG25.405405405405405No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAAAAAACGGAGTGGTGCCATTCAGGTA25.405405405405405No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT25.405405405405405No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGGGGCACCAGCAGCGGGTAGCCGGCAA25.405405405405405No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG25.405405405405405No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG25.405405405405405No Hit
TTTCAGGTAGGTGGACTTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTT12.7027027027027026RNA PCR Primer, Index 32 (96% over 26bp)
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTT12.7027027027027026RNA PCR Primer, Index 32 (96% over 26bp)
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCACTGCTGCAGAACTTGATAGCCACACC12.7027027027027026No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATCAGCAGCACTCTGCACAGCATCATCCAT12.7027027027027026No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTTCCGAGCACACGAGACACCATTCAATCTCGTATGCCGTCTT12.7027027027027026Illumina PCR Primer Index 8 (95% over 21bp)
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGTAGTAGAAAAAACGGAGTGGTGCCATTCAGGTA12.7027027027027026No Hit
ACATCAGCCTCTTTATTCTCAGCATGGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACTACCTGA12.7027027027027026No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTT12.7027027027027026RNA PCR Primer, Index 32 (96% over 26bp)
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACAACTTCTGCCCCTGCGCACATG12.7027027027027026No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTGTT12.7027027027027026No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTACGAGCCCACGAGACACCATTCAATGTCGTATGCCGTCTT12.7027027027027026No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCNCCTGCGCACATG12.7027027027027026No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCACACGAGACACAATTAAAACTCGTATGACGTA12.7027027027027026No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCAACTAGACACGATTCAAGGTCGTATGCCGGCGT12.7027027027027026No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGTGGCGGTATGCTGCGCGGGGCCCGCGCCCC12.7027027027027026No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCT12.7027027027027026RNA PCR Primer, Index 32 (96% over 28bp)
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTACCACCTTCCCATACGCAACACTCTGCACAGCATCATCCAT12.7027027027027026No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCTGGAGGACCGCATTGACTACCTGA12.7027027027027026No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT12.7027027027027026No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAGCTGGAGGACCGCATTGACTACCTGA12.7027027027027026No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCGTGTAGAGCAGCGTGAAGATGCCGAT12.7027027027027026No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGNTATCAGCCAAGC12.7027027027027026No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGAAACCATTCACTCTCGTATGCCGTCTTCT12.7027027027027026TruSeq Adapter, Index 15 (95% over 22bp)
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATAACAGTTGCTTTGTTGACAACAGTCA12.7027027027027026No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCGGGAGGCTGCGCGGGGCCCGCGCCCC12.7027027027027026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)