FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002294514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606526
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGC78490.4885697461478993No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG42780.26628887425413594RNA PCR Primer, Index 47 (96% over 30bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGC31470.1958885197002725No Hit
ACACATCTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG17980.11191851236768031RNA PCR Primer, Index 47 (96% over 30bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG17320.10781026886586337No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTG17280.10756128441120778No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTG16990.10575614711495487No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)