FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002294519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235567
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG23330.9903764109573922Illumina PCR Primer Index 1 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGC13850.5879431329515594No Hit
ACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG8070.3425776955176234Illumina PCR Primer Index 1 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG7160.3039474968904813Illumina Paired End PCR Primer 2 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG5400.22923414569952497Illumina PCR Primer Index 1 (96% over 28bp)
CATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG5250.22286653054120484Illumina PCR Primer Index 1 (96% over 28bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTC5230.22201751518676213No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGC4910.2084332695156792No Hit
CACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG3650.15494530218579003RNA PCR Primer, Index 36 (96% over 30bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTC3540.15027571773635526No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAAC3230.13711597974249365No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATG2950.12522976478029604No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGA2860.12140919568530398Illumina Paired End PCR Primer 2 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG2750.1167396112358692No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTC2470.1048533962736716No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACC2470.1048533962736716No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)