FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC78029.44507361268403No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC33112.495281238203097No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAA1796.757266893167232No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG732.7557568893922237No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC692.6047565118912797No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC682.5670064175160436No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG602.2650056625141564No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG542.0385050962627407No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC481.8120045300113252No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC451.698754246885617No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG361.3590033975084939No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT341.2835032087580218No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG311.1702529256323142No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG291.0947527368818422No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA281.0570026425066061No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG250.9437523593808984No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT240.9060022650056626No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC230.8682521706304266No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT220.8305020762551907No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT180.6795016987542469No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTA160.604001510003775No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC140.5285013212533031No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT120.4530011325028313No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCCACGTATATCTCGT100.3775009437523594No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC90.33975084937712347No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGGATAACAATCTCGT90.33975084937712347No Hit
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG80.3020007550018875No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA80.3020007550018875No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGT80.3020007550018875No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACA80.3020007550018875No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC80.3020007550018875No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA70.26425066062665153No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCG60.22650056625141565No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG50.1887504718761797No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGT50.1887504718761797No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAAGCGTGTATCTCGT50.1887504718761797No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACAGACA50.1887504718761797No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTTCGAGCCCACGAGAC50.1887504718761797No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT50.1887504718761797No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACAGACA50.1887504718761797No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTCCGAGCCCACGAGACA40.15100037750094375No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAACCCACGAGAC40.15100037750094375No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTTGGAAGATCTCGT40.15100037750094375No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC40.15100037750094375No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCG40.15100037750094375No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG40.15100037750094375No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC40.15100037750094375No Hit
CAACCAAAAGGACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC40.15100037750094375No Hit
CAACCAAAAGAACAGCCTGTCTCTTATATACATCTCCGAGCCCACGAGAC40.15100037750094375No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACACCTCCGAGCCCACGAGAC40.15100037750094375No Hit
CAACCAAAAGAACAGCCTGTCTCTATACACATCTCCGAGCCCACGAGACA40.15100037750094375No Hit
CAAGCAATCAAAGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAA40.15100037750094375No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT40.15100037750094375No Hit
CAATCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC30.11325028312570783No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG30.11325028312570783No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCAC30.11325028312570783No Hit
CAAGCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACA30.11325028312570783No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT30.11325028312570783No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGACCACGAGAC30.11325028312570783No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGCC30.11325028312570783No Hit
GTCATTGTCACTGGTCATCTCCGAGCCCACGAGACAAGCGTGTATCTCGT30.11325028312570783No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGAC30.11325028312570783No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAGAGACA30.11325028312570783No Hit
AAACTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC30.11325028312570783No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATT30.11325028312570783No Hit
CTTCTTGTTTTGGGGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG30.11325028312570783No Hit
ATAGCAGAATCATCGTGGTCTCCTACAACTTCTGAAGGCTGTCTCTTATA30.11325028312570783No Hit
CAAGCAAAAGAACAGCCTGTCTCTGATACACATCTCCGAGCCCACGAGAC30.11325028312570783No Hit
CAAGCAATCAAGGCCAGTCTTTATACACATCTCCGAGCCCACGAGACAAG30.11325028312570783No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGAC30.11325028312570783No Hit
CAACCAAAAGAACAGCCTGTCTCTTATAAACATCTCCGAGCCCACGAGAC30.11325028312570783No Hit
CAACCAAAAGAACAGCCTGTCTCTTAACACATCTCCGAGCCCACGAGACA30.11325028312570783No Hit
CCTGACTCACATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCG30.11325028312570783No Hit
CAACCAAAAGAACAGCCTGTCTCTTTTACACATCTCCGAGCCCACGAGAC30.11325028312570783No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGCC30.11325028312570783No Hit
CCTGACTCACGTTTCCTTATTGCTGGGATGTTCACAGAGCCAACGTCCTG30.11325028312570783No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACAAGAC30.11325028312570783No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)