FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4303
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC123428.677666744132No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC65115.128979781547757No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGA51311.92191494306298No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC49411.48036253776435No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC932.161282825935394No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC811.8824076225888913No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC581.3478968161747618No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG390.906344410876133No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG290.6739484080873809No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGGATAACAATCTCGT260.6042296072507553No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA250.5809900069718801No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGT220.5112712061352545No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC200.46479200557750405No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG190.4415524052986289No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTA160.37183360446200325No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG150.348594004183128No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT120.27887520334650245No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT120.27887520334650245No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCCACGTATATCTCGT120.27887520334650245No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC110.2556356030676272No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC110.2556356030676272No Hit
CCTGACTCACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCG100.23239600278875203No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACG100.23239600278875203No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC100.23239600278875203No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCAAGCGATATCTCGT100.23239600278875203No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCG90.20915640250987683No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCG80.18591680223100163No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGCCCACGAGAC80.18591680223100163No Hit
GTAGTGCTGCTCATCGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG70.16267720195212643No Hit
CAACCAAAAGAACAGCCTGTCTCTTTTACACATCTCCGAGCCCACGAGAC70.16267720195212643No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTTGGAAGATCTCGT60.13943760167325123No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG60.13943760167325123No Hit
CCTGACTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGT60.13943760167325123No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT60.13943760167325123No Hit
ACACCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG60.13943760167325123No Hit
CAAGCAATCAAGGCCAGTCCCTTATACACATCTCCGAGCCCACGAGACGA60.13943760167325123No Hit
CTTCAGTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA60.13943760167325123No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGAC60.13943760167325123No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT60.13943760167325123No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT60.13943760167325123No Hit
CAAGCAATCAAAGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACGA60.13943760167325123No Hit
CAACCAAAAGAACAGCCTGTCTTTTATACACATCTCCGAGCCCACGAGAC60.13943760167325123No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG50.11619800139437601No Hit
CAAGCAATCAAGGCCAGTCTTTATACACATCTCCGAGCCCACGAGACGAT50.11619800139437601No Hit
CAAGCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC50.11619800139437601No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG50.11619800139437601No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTCCGAGCCCACGAGACG50.11619800139437601No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC50.11619800139437601No Hit
CAACCAAAAGAACGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC50.11619800139437601No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGAC50.11619800139437601No Hit
CAACCAAAAGAACAGCCTGTCTCTCATACACATCTCCGAGCCCACGAGAC50.11619800139437601No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT50.11619800139437601No Hit
CAACCAAAAGAACAGCCTGTCTCTTATAAACATCTCCGAGCCCACGAGAC50.11619800139437601No Hit
GGTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTG50.11619800139437601No Hit
CAAGCAATCAAGGCCAGTCTGTCTCTTATACACATCTCCGAGCCCACGAG50.11619800139437601No Hit
CAAGCAATCAAGGCCAGTCTCTTATACAAATCTCCGAGCCCACGAGACGA50.11619800139437601No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCGAGCCCACGAGACG50.11619800139437601No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC50.11619800139437601No Hit
CAACCAAAAGAACAGCCTGTCTCTTAACACATCTCCGAGCCCACGAGACG50.11619800139437601No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)