FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8384
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC381845.53912213740458No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCA97911.677003816793894No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC7569.017175572519085No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC4545.415076335877863No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC2232.6598282442748094No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCAAGCGATATCTCGT630.7514312977099237No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGT490.5844465648854962No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTA480.5725190839694656No Hit
CTTCTTGTTTTGGAGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG370.44131679389312983No Hit
CAACCAAAAGAACAGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC310.36975190839694655No Hit
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCA260.3101145038167939No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC250.2981870229007634No Hit
CAACCAAAAGAACAGCCTGTCTCTATACACATCTCCGAGCCCACGAGACC230.2743320610687023No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGGACC220.26240458015267176No Hit
CAACCAAAAGAACAGCCTGTCTTTATACACATCTCCGAGCCCACGAGACC220.26240458015267176No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCGAGCCCACGAGACC220.26240458015267176No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG200.2385496183206107No Hit
CAACCAAAAGAACAGCCTGTCCCTTATACACATCTCCGAGCCCACGAGAC200.2385496183206107No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGAC200.2385496183206107No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACAAATCTCCGAGCCCACGAGAC190.22662213740458015No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACAGACC180.21469465648854963No Hit
ACACCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG170.20276717557251908No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTTCGAGCCCACGAGAC160.19083969465648853No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC160.19083969465648853No Hit
ATAGCAGAATCATCGTGGTCTCCTACAACTTCTGAAGGCTGTCTCTTATA150.17891221374045801No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTCCGAGCCCACGAGACC150.17891221374045801No Hit
CAACCAAAAGAACAGCCTGTCTCTTTACACATCTCCGAGCCCACGAGACC140.16698473282442747No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCTCACGAGAC140.16698473282442747No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACACCTCCGAGCCCACGAGAC130.15505725190839695No Hit
CCTGACTCTTATACACATCTCCGAGCCCACGAGACCAAGCGATATCTCGT130.15505725190839695No Hit
CAACCAAAAGAACAGCCTGTCTCTTATATACATCTCCGAGCCCACGAGAC130.15505725190839695No Hit
CAACCAAAAGAACAGCCTGTCTTTTATACACATCTCCGAGCCCACGAGAC130.15505725190839695No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG120.1431297709923664No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCTGAGCCCACGAGAC120.1431297709923664No Hit
CAACCAAAAGAACAGCCTGTCTCTTATAACATCTCCGAGCCCACGAGACC120.1431297709923664No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT110.13120229007633588No Hit
CAAGCAATCAAGGCCAGTCCCTTATACACATCTCCGAGCCCACGAGACCA110.13120229007633588No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATTTCCGAGCCCACGAGAC110.13120229007633588No Hit
CAAGCAATCAAGGCCAGTCTTTATACACATCTCCGAGCCCACGAGACCAA110.13120229007633588No Hit
ACATCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG110.13120229007633588No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCATGAGAC100.11927480916030535No Hit
CAACCAAAAGGACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC100.11927480916030535No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCCACGTATATCTCGT100.11927480916030535No Hit
CAAGCAATCAAAGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCA100.11927480916030535No Hit
CCTGACTCACATCTCCGAGCCCACGAGACCAAGCGATATCTCGTATGCCG100.11927480916030535No Hit
CTTCTTGTTTTGGGGCACTCAGTTGTCTGTCTCTTATACACATCTCCGAG100.11927480916030535No Hit
CAACCAAAAGAACAGCCTGTCTCTTAACACATCTCCGAGCCCACGAGACC100.11927480916030535No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG90.10734732824427481No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACATATCTCCGAGCCCACGAGAC90.10734732824427481No Hit
CAACCAAAAGAACAGCCTGTCTCTTTTACACATCTCCGAGCCCACGAGAC90.10734732824427481No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGCC90.10734732824427481No Hit
CAACCAAAAGAACGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC90.10734732824427481No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)