FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC12723.049001814882033No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCG458.166969147005444No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCC325.807622504537205No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGGATAACAATCTCGT285.081669691470054No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTT274.900181488203267No Hit
TCTCGTGCTCGCAGATGCCGCCCAGCGGCTCCGGGGCGGCAGGTGGGGCG213.8112522686025407No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGGGACCG203.6297640653357535No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCGC162.9038112522686026No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG152.722323049001815No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGC122.1778584392014517No Hit
CAGTGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT122.1778584392014517No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC101.8148820326678767No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT101.8148820326678767No Hit
AAAGTCTCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCAGGAGGAGAA81.4519056261343013No Hit
TGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA71.2704174228675136No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCACCTTCCCATAAG61.0889292196007259No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTACAAT61.0889292196007259No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCG61.0889292196007259No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCACAGCAATAAG61.0889292196007259No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAAT50.9074410163339384No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGA50.9074410163339384No Hit
GGTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTG40.7259528130671506No Hit
TTTCAGGTAGGTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC40.7259528130671506No Hit
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAG40.7259528130671506No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCC40.7259528130671506No Hit
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAG40.7259528130671506No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGATGCACAATCTCGT40.7259528130671506No Hit
GAAGATGCTGATTCTCTTGCAGGAACTCACCCACTCATCCTGCAAAGCTT40.7259528130671506No Hit
ATGCTCAGTTGTAGGTGATTCTCACTTTACAACTTTTGATGGTCGACATT30.5444646098003629No Hit
CTGTTTTCATCCCACAGGTTGTTATTTTGTACTTTGAGCCAAGCACATTT30.5444646098003629No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC30.5444646098003629No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGGCACCGGGTCATTCTTTT30.5444646098003629No Hit
GTCCAGGGTGGGCATGGATTGCAGGGCCGCGGGCACGGGGGGCACTGCGT20.3629764065335753No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGC20.3629764065335753No Hit
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGTGGTAGGGCCGG20.3629764065335753No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCG20.3629764065335753No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG20.3629764065335753No Hit
TGGAATAAGTTCAGTCTCACAGCATTTTACTGACACCGGGTCATTCTTTT20.3629764065335753No Hit
GAAGCAGTGGATCTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCG20.3629764065335753No Hit
AACTGTGCTTACACTGACTCCGAGCCCAGGAGACGGATAACACACTCGTA10.18148820326678766No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG10.18148820326678766No Hit
AGCCAAGGGTCTAAAGAAGTGGAAGTCGCTCTTGTTCGGAAAGACAAAGG10.18148820326678766No Hit
CAAGCAAAAGAACAGCCTGTCTCTTGTACACATCTCCGAGCCCACGAGAA10.18148820326678766No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCCGAGACCCA10.18148820326678766No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGGATAACAACCTCGT10.18148820326678766No Hit
ATAGCAGAATCATCGTGGTCTCCAGAAGTGCCCACGTTGCTCTTGCCGCT10.18148820326678766No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCATCGACCATCTCGT10.18148820326678766No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCAAGCGAGCTCGTAT10.18148820326678766No Hit
CAAGCAATCAAGCCAGTCCTTATACACATCTCCGAGCCCACGAGACCCAC10.18148820326678766No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG10.18148820326678766No Hit
CAATGACATGTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCT10.18148820326678766No Hit
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCC10.18148820326678766No Hit
AAAGTCTCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10.18148820326678766No Hit
TGTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTG10.18148820326678766No Hit
CTCTCAGCTCCGAGCCCACGAGACGGATAACAACTTCGTATGCCGTCTTC10.18148820326678766No Hit
CATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAA10.18148820326678766No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGGATAACAAACTCGTATGCCG10.18148820326678766No Hit
GGGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCATTTGCACCCG10.18148820326678766No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGGATAACAATATCGTATGCCG10.18148820326678766No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTAGTGGCCACTTCTGCAAT10.18148820326678766No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCAGTAGCGCGCCATTTGCACCCGC10.18148820326678766No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGGATAACAAACTCGT10.18148820326678766No Hit
GGCTGTGGTGTGAGTCCGGGTGGCGGCCGTAGCGCGCCACTTGCACCCGC10.18148820326678766No Hit
GTCATTGTCACTGGTCAGCTCCGAGCCCACGAGACGGATAACAATCTCGT10.18148820326678766No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGGATAACAATCCCGT10.18148820326678766No Hit
ACATCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACG10.18148820326678766No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGGATAACAAGCTCGT10.18148820326678766No Hit
AACTGCAGCTTACACTGACTCCGAGCCCACGAGACGGATAACAATCTCGT10.18148820326678766No Hit
GTAAAAGTTGAAATGTCAGAGCCCACGAGACGGATAACAACCTCGTATGC10.18148820326678766No Hit
GGCTGTGGTGTGAGTCCGAGCCCACGAGACGGATCACAATCTCGTATGCC10.18148820326678766No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGTGACGGATAACAATCTCGT10.18148820326678766No Hit
GTTTCCAGGTCAAGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCC10.18148820326678766No Hit
CAACCAAAAGAACCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA10.18148820326678766No Hit
AACTGTAGTTTACACTGACTCCGAGCCTACGAGACGGATAACAATTTCGT10.18148820326678766No Hit
GGAATGAGGTCAAGAGGATCTGGTGAGCCCTGGCAGAGAGGACTTATATA10.18148820326678766No Hit
GTCATTGTCACTGGTCAGCTCCAACACCTTCTGCTGCGTCTCCACGTTGC10.18148820326678766No Hit
GTCATTGTCACTGGTCAGCTCCAGCACATTCTGCCGCGCCTCCACGTTGC10.18148820326678766No Hit
CTTCTTGCTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGT10.18148820326678766No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGGATAACAATTTCGTATGCCG10.18148820326678766No Hit
TTTCAGGTCAGAGCCCACGAGACGGATAACAATCTCGTATGCCGTCTTCT10.18148820326678766RNA PCR Primer, Index 36 (95% over 24bp)
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAAACCA10.18148820326678766No Hit
GTAGTGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCGGCGACCC10.18148820326678766No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCATAGCCCTCCTTGGTCACTGT10.18148820326678766No Hit
CAACCAAAAGAACAGCCTGTCTCTAATACACATCTCTGAGCCCACGCGAC10.18148820326678766No Hit
CCTGACTCACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG10.18148820326678766No Hit
CCTGACTCACGTTTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCG10.18148820326678766No Hit
GTCATTGTCATGGTCATCTCCGAGCCCACGAGACAAGCGTGTAACTCGTA10.18148820326678766No Hit
CATAGTTACCTTCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCG10.18148820326678766No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10.18148820326678766No Hit
GTCATTGTCACTGGTTAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGC10.18148820326678766No Hit
AAAGTCCTGACGTGGATGAGTTTGGGAGTGTGGAAGCTCGGGAGGAGAAA10.18148820326678766No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCAAGCCCACGAGAC10.18148820326678766No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGTCTCCACGTTGT10.18148820326678766No Hit
CCTGACTCTTATACACATCTCCGAGCCCACGAGCGGATAACAATCTCGTA10.18148820326678766No Hit
TAAAATAGTAGCCCTCTCGCACTCACCAGGTCCGGTCGGTAGTACCTGTG10.18148820326678766No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACCAAGCGATATCTCGT10.18148820326678766No Hit
AACTGTAGCTTACACTGCTCCGAGCCCACGAGACGGATAACAATCTCGTA10.18148820326678766No Hit
CCTGGCTCACGTTTCCGAGCCCACGAGACGGATAACAATCTCGTATGCCG10.18148820326678766No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGCCTCCACGTTGC10.18148820326678766No Hit
AACTGTAGCTTACACTGACTCCGTGCCCACGAGACGGATAACAATCACGA10.18148820326678766No Hit
GAAGCAGTGGATCTCCGAGCCCACGAGACAAGCGTGTATCTCGTATGCCG10.18148820326678766No Hit
TTTCAGGTAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCC10.18148820326678766No Hit
TTTCAGGTCAGAGCTGCTCGTACAGGTAGCAGGCCACCACAATGCTGGCG10.18148820326678766No Hit
GTCATTGTCACTGGTCAGCTCCAGCACCTTCTGCTGCGGCTCCACGTTGC10.18148820326678766No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGGATAACAACCTTGT10.18148820326678766No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAGAGACG10.18148820326678766No Hit
AACTGTAGCTTACACTGACTCCATGGAACAGTTTCTCCCCAATGAGACGA10.18148820326678766No Hit
AACTGTAGCTTACATGACTCCGAGCCCACGAGACGTGCATTCATCTCGTA10.18148820326678766No Hit
GCACAGGTCGTATACGCATTGGCGGACGTACTCAGAGGGACTCAGCACCG10.18148820326678766No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGCGAC10.18148820326678766No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACAAGCGTGTATCTCGT10.18148820326678766No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAACG10.18148820326678766No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)