FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002294598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323386
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGC253911.9186390063065502No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGC70760.5346890476399176No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG34170.2582013108798189No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGC33410.25245846638849134No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGC29430.22238409655232866No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC27370.2068179654310987No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCA25210.19049619687679936No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC25200.19042063313349242No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG24790.1873225196579078TruSeq Adapter, Index 7 (96% over 28bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC23310.17613908564848046No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGC22610.1708496236169946No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC19900.15037184918081345No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGC19790.14954064800443712No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCT17630.13321887945013774No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG15020.11349674244702604No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)