FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002294610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280436
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGC75690.5911267724431365No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG58780.45906238187617343Illumina PCR Primer Index 6 (96% over 27bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTC26480.20680455719770452No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG24360.19024769687825083Illumina PCR Primer Index 6 (96% over 27bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGC20470.159867420160008No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTC19340.15104230121614826No Hit
ACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG19020.14854315248868352Illumina PCR Primer Index 6 (96% over 27bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTC18130.14159239509042235No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG17850.13940563995389071No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCAC15740.1229268780321703No Hit
ATACACATCTCCGAGCCCACGAGACCCACGTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG13850.10816628086058187Illumina PCR Primer Index 6 (96% over 27bp)
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCACGT13420.10480804975805116No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)