FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002294619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2078437
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC43243320.80568234687893No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC1988879.569065600737476No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC1670018.03493201862746RNA PCR Primer, Index 32 (95% over 24bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC1507067.250929424370332RNA PCR Primer, Index 32 (95% over 24bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC1249726.012787493679145RNA PCR Primer, Index 32 (95% over 24bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC872424.1974810879521485RNA PCR Primer, Index 32 (95% over 24bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTC647453.115081188412254No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAA523912.5206922317106555No Hit
GGTCGGTAGTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGC229911.106167759715594RNA PCR Primer, Index 32 (96% over 30bp)
CATCCGCAGCGTCATCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC229101.1022706004560159RNA PCR Primer, Index 32 (95% over 24bp)
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTT149780.7206376714810215RNA PCR Primer, Index 32 (96% over 26bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGC138510.6664142333878775No Hit
GTCCAGGGTGGACATGGGCACACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC135810.6534237025226167No Hit
CTAGTTTCCTCTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC111330.5356428893442524RNA PCR Primer, Index 32 (95% over 24bp)
CTGCTTCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTG99960.48093832047832097RNA PCR Primer, Index 32 (96% over 33bp)
GTCCAGGGTGGGCATGGGCACACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC99100.4768005958323491No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATC83820.40328381375042877No Hit
TCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC79400.3820178335932241RNA PCR Primer, Index 32 (95% over 24bp)
GCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC70780.3405443609789472RNA PCR Primer, Index 32 (95% over 24bp)
CATCCGCAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT70180.33765757634222254RNA PCR Primer, Index 32 (95% over 23bp)
CATCCATAGCGTCCTCATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT68230.32827552627286755RNA PCR Primer, Index 32 (95% over 23bp)
CCCGATGAGCAGCACCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC66340.319182154667185RNA PCR Primer, Index 32 (95% over 24bp)
CCCGATGAGCAGCCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT66220.3186047977398401RNA PCR Primer, Index 32 (95% over 23bp)
GCTCTCAGCTCCGTAGTGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG65800.31658404849413285RNA PCR Primer, Index 32 (95% over 22bp)
GTTTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTAT65590.31557367387127927No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGC61550.2961359906506668No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTC58110.279585092066779No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTC56750.2730417135568699No Hit
GTTTCCACGTCAGAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG56700.2728011481704762RNA PCR Primer, Index 32 (95% over 22bp)
GTTTCCAGGTCAAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATC54630.26284174117377623No Hit
GGTCATTGTCACTGGTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGT51380.2472049910581846RNA PCR Primer, Index 32 (95% over 23bp)
CATCCGCAGCGTCCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC49170.23657200097958225RNA PCR Primer, Index 32 (95% over 24bp)
CATCCATAGCGTCCTCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC46220.2223786431823529RNA PCR Primer, Index 32 (95% over 24bp)
AAAGTTGAAATGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTC45900.2208390247094331No Hit
CAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTG45760.22016544162753066RNA PCR Primer, Index 32 (96% over 33bp)
CTTCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC43020.20698245845315494RNA PCR Primer, Index 32 (95% over 24bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTC42630.20510604843928396No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTC41780.20101643687059073No Hit
GTTTCCAGGTCAAGGGTAACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAA39040.18783345369621499No Hit
GCTCTCAGCTCCGTAAAGGTGGAGCTCATCTTCATTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCA37730.18153064057269958No Hit
CTAGTTTCCTTTGGCTTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC36090.17364009589898563RNA PCR Primer, Index 32 (95% over 24bp)
CTTCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC35890.17267783435341078RNA PCR Primer, Index 32 (95% over 24bp)
GTTTCCAGGTCAGAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCG35890.17267783435341078RNA PCR Primer, Index 32 (95% over 22bp)
AAAGTTGAAATGTCAGGGTCATGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTC34580.16637502122989534No Hit
CATCCGCAGCGTCATCAACTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC30590.14717790339567666RNA PCR Primer, Index 32 (95% over 24bp)
AAAGTTGAAATATCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTC30350.1460231895409868No Hit
CATCCGTAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTC29210.14053829873121004RNA PCR Primer, Index 32 (95% over 24bp)
GGCTGTGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC29150.1402496202675376No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAAGCTCGTATGC26820.12903927326159031No Hit
GTCCAGGGTGGAGCTCATCTTCATTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGT26750.1287024817206391No Hit
CATCCGCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGCTTG25190.12119684166515511RNA PCR Primer, Index 32 (96% over 33bp)
GACCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGC23890.11494214161891846No Hit
GGCTGTGGTGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATGCCGTCTTCTGC23270.11195913082763635RNA PCR Primer, Index 32 (96% over 30bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATGTCGTATGC22800.10969781619553541No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTATG22770.10955347696369917No Hit
GTTTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATCTCGTAT21970.10570443078139968No Hit
CCCGATGAGCAGCCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCATTCAATATCGTATGCCGTCTT21580.10382802076752869No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)