FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002294638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518625
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGC284841.1309345376941784No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG120930.4801429351332573RNA PCR Primer, Index 27 (96% over 30bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC102560.40720631296838555RNA PCR Primer, Index 27 (95% over 21bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGC84780.3366122388207851No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC84120.3339917613777359RNA PCR Primer, Index 27 (95% over 21bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC77930.3094148592982282RNA PCR Primer, Index 27 (95% over 21bp)
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC57130.22683011563849323RNA PCR Primer, Index 27 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG55090.21873045808725RNA PCR Primer, Index 27 (96% over 30bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG49700.1973298923023475No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG44950.17847039555312919RNA PCR Primer, Index 27 (96% over 30bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAC41290.16393865700531043No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAC41050.16298575611692886No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC39780.15794332224924315No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG38530.15298029678892253RNA PCR Primer, Index 27 (96% over 30bp)
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC35580.14126755670256588No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC33140.13157973100402007No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTA28720.11403047297632637No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)