FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69109
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG20392.9504116685236363TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG10471.5149980465641233RNA PCR Primer, Index 3 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG6650.9622480429466495TruSeq Adapter, Index 3 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG6200.8971335137246957RNA PCR Primer, Index 3 (96% over 28bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG5970.8638527543445861TruSeq Adapter, Index 3 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGC5760.8334659740410076No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG4750.6873200306761782TruSeq Adapter, Index 3 (96% over 28bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC2710.39213416486998803No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC1740.2517761796582211No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGC1440.2083664935102519No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC1200.1736387445918766No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC1110.16061583874748586No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG1010.14614594336482947TruSeq Adapter, Index 3 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG980.14180497475003256TruSeq Adapter, Index 3 (96% over 28bp)
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA940.13601701659697TruSeq Adapter, Index 3 (96% over 28bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAA920.13312303752043872No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG900.13022905844390747No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG850.12299411075257925No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG840.12154712121431363No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGG820.11865314213778234RNA PCR Primer, Index 3 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG750.10852421536992288TruSeq Adapter, Index 3 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAG740.10707722583165724No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAG730.1056302362933916No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)