FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG1057.954545454545454TruSeq Adapter, Index 11 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG665.0RNA PCR Primer, Index 11 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG513.8636363636363633TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG483.6363636363636362RNA PCR Primer, Index 11 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG302.272727272727273TruSeq Adapter, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG302.272727272727273TruSeq Adapter, Index 11 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGC261.9696969696969695No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG181.3636363636363635TruSeq Adapter, Index 11 (96% over 28bp)
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG181.3636363636363635No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTA171.2878787878787878No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA171.2878787878787878TruSeq Adapter, Index 11 (96% over 28bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGC161.2121212121212122No Hit
GTACTACCGACCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG90.6818181818181818TruSeq Adapter, Index 11 (95% over 24bp)
GTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG90.6818181818181818TruSeq Adapter, Index 11 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGAT90.6818181818181818No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG80.6060606060606061No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGGGGG70.5303030303030304TruSeq Adapter, Index 11 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGGGGGGG60.45454545454545453TruSeq Adapter, Index 11 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGGGGGGGGGG60.45454545454545453TruSeq Adapter, Index 11 (96% over 28bp)
GGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT60.45454545454545453No Hit
GTACTACCGACCCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA60.45454545454545453No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGG60.45454545454545453RNA PCR Primer, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGTGGGG60.45454545454545453TruSeq Adapter, Index 11 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT60.45454545454545453No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGG50.3787878787878788RNA PCR Primer, Index 11 (96% over 28bp)
GTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT50.3787878787878788TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGGGG50.3787878787878788RNA PCR Primer, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGGGGG50.3787878787878788TruSeq Adapter, Index 11 (96% over 28bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGG50.3787878787878788TruSeq Adapter, Index 11 (96% over 28bp)
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC40.30303030303030304No Hit
GTACTACCGACCCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCG40.30303030303030304No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATTGGGG40.30303030303030304TruSeq Adapter, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGG40.30303030303030304TruSeq Adapter, Index 11 (96% over 28bp)
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGA40.30303030303030304No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT40.30303030303030304No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG40.30303030303030304TruSeq Adapter, Index 11 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGGG40.30303030303030304TruSeq Adapter, Index 11 (96% over 28bp)
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC40.30303030303030304No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGG40.30303030303030304TruSeq Adapter, Index 11 (96% over 28bp)
TGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA30.22727272727272727TruSeq Adapter, Index 11 (96% over 28bp)
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA30.22727272727272727TruSeq Adapter, Index 11 (96% over 28bp)
CTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG30.22727272727272727TruSeq Adapter, Index 11 (96% over 28bp)
GCTCTCAGCTCCGTAAAGGTGGAGCTCATCTTCATTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTA30.22727272727272727No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTAC30.22727272727272727No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCC30.22727272727272727No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG30.22727272727272727No Hit
GTCCAGGGTGGACATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT30.22727272727272727TruSeq Adapter, Index 11 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGG30.22727272727272727RNA PCR Primer, Index 11 (96% over 28bp)
GTGTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT30.22727272727272727No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTA30.22727272727272727No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGGGGGGG30.22727272727272727RNA PCR Primer, Index 11 (96% over 28bp)
AAAGTTGAAATGTCAGGGTCACGTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT30.22727272727272727No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGGGGGGG30.22727272727272727RNA PCR Primer, Index 11 (96% over 28bp)
GGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAA30.22727272727272727TruSeq Adapter, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG30.22727272727272727Illumina PCR Primer Index 9 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGGGGGGG30.22727272727272727RNA PCR Primer, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGGGG30.22727272727272727TruSeq Adapter, Index 11 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGG30.22727272727272727RNA PCR Primer, Index 11 (96% over 28bp)
GACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCG30.22727272727272727No Hit
TACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGG30.22727272727272727TruSeq Adapter, Index 11 (96% over 28bp)
GGTCCAGGGTGGGCATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATC30.22727272727272727No Hit
TGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT20.15151515151515152No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG20.15151515151515152TruSeq Adapter, Index 11 (96% over 28bp)
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT20.15151515151515152No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCA20.15151515151515152No Hit
AAAGTTGAAATGTCAGGGCCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTA20.15151515151515152No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG20.15151515151515152Illumina Single End Adapter 1 (96% over 27bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGGGGGGGG20.15151515151515152TruSeq Adapter, Index 11 (96% over 28bp)
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACC20.15151515151515152No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAATAGGGGG20.15151515151515152TruSeq Adapter, Index 11 (96% over 28bp)
GGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC20.15151515151515152TruSeq Adapter, Index 11 (96% over 25bp)
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA20.15151515151515152TruSeq Adapter, Index 11 (96% over 28bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCC20.15151515151515152No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA20.15151515151515152No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTC20.15151515151515152No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT20.15151515151515152No Hit
GTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG20.15151515151515152No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG20.15151515151515152Illumina PCR Primer Index 9 (96% over 26bp)
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC20.15151515151515152No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGGG20.15151515151515152RNA PCR Primer, Index 11 (96% over 28bp)
GGGTAACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT20.15151515151515152No Hit
GAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCG20.15151515151515152No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACGGGG20.15151515151515152TruSeq Adapter, Index 11 (96% over 28bp)
GAACCGGAAGAGCAGCGCCTTCCAGACCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCA20.15151515151515152No Hit
GTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAA20.15151515151515152TruSeq Adapter, Index 11 (96% over 28bp)
GTCCAGGGTGGACATGGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT20.15151515151515152No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTA20.15151515151515152No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC20.15151515151515152No Hit
CGTGCCCATGCCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG20.15151515151515152No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGAGGGGGGGG20.15151515151515152RNA PCR Primer, Index 11 (96% over 28bp)
CCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT20.15151515151515152No Hit
GTCCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG20.15151515151515152TruSeq Adapter, Index 11 (96% over 28bp)
GGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG20.15151515151515152TruSeq Adapter, Index 11 (95% over 24bp)
GTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC20.15151515151515152TruSeq Adapter, Index 11 (95% over 22bp)
GTTTCCAGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG20.15151515151515152TruSeq Adapter, Index 11 (96% over 28bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCC20.15151515151515152No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACAC20.15151515151515152No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCC20.15151515151515152No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCC20.15151515151515152No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGAGGGGGGG20.15151515151515152TruSeq Adapter, Index 11 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG20.15151515151515152No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGCGGG20.15151515151515152TruSeq Adapter, Index 11 (96% over 28bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG20.15151515151515152Illumina PCR Primer Index 9 (96% over 26bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTA20.15151515151515152No Hit
GTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT20.15151515151515152No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACAT20.15151515151515152No Hit
GTTTCCAGGTCAAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT20.15151515151515152TruSeq Adapter, Index 11 (95% over 21bp)
AAAGTTGAAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC20.15151515151515152TruSeq Adapter, Index 11 (96% over 25bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATAGGGGGGGGGG20.15151515151515152TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG20.15151515151515152Illumina PCR Primer Index 9 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)