FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002294660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306223
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG58290.44624845834133986TruSeq Adapter, Index 1 (96% over 29bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGC57950.4436455337258646No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC36000.27560378281503234No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC35140.2690199146700066No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC25080.19200396869447253No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG23850.18258750611495894RNA PCR Primer, Index 1 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG19270.1475245804123798RNA PCR Primer, Index 1 (96% over 29bp)
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGAT18260.1397923631722914No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC16920.12953377792306522No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG16780.1284619854343401No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGA16560.12677774009491488No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG15100.11560047556963857TruSeq Adapter, Index 1 (96% over 29bp)
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAG14350.10985873009432538No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCT14290.10939939045630034No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC14010.1072558054788501No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCA13940.10671990923448753No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGC13200.10105472036551187No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)