FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5510
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAGTCTGGTGTTCCTGTTCATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCG380.6896551724137931No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC330.5989110707803993No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC320.5807622504537204No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG320.5807622504537204No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGA290.5263157894736842No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC280.5081669691470054No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGA270.4900181488203267No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG260.47186932849364793No Hit
GAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCG250.4537205081669692No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG250.4537205081669692No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA240.43557168784029043No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGA240.43557168784029043No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA220.3992740471869328No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG220.3992740471869328TruSeq Adapter, Index 10 (96% over 28bp)
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAG220.3992740471869328No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC200.3629764065335753No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACC200.3629764065335753No Hit
CTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG190.3448275862068966No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC190.3448275862068966No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATC190.3448275862068966No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC190.3448275862068966No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGA180.32667876588021777No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGA180.32667876588021777No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAG180.32667876588021777No Hit
CTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA170.308529945553539No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAG160.2903811252268602No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATC160.2903811252268602No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCA160.2903811252268602No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG160.2903811252268602No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCC160.2903811252268602No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGG150.27223230490018147No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGA150.27223230490018147No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCAC150.27223230490018147No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA150.27223230490018147No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC140.2540834845735027No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCC140.2540834845735027No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGA140.2540834845735027No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC140.2540834845735027No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG140.2540834845735027No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGA140.2540834845735027No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGA140.2540834845735027No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCAC130.23593466424682397No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCAC130.23593466424682397No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACG130.23593466424682397No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG130.23593466424682397No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA130.23593466424682397No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT130.23593466424682397No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG120.21778584392014522RNA PCR Primer, Index 10 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGC120.21778584392014522No Hit
TTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG120.21778584392014522No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGA120.21778584392014522No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCC120.21778584392014522No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC110.1996370235934664No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCCACGAG110.1996370235934664No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCC110.1996370235934664No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG110.1996370235934664No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGA110.1996370235934664No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCAC110.1996370235934664No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG100.18148820326678766No Hit
GCCAAGAAAGCACGAGCATCAGCCGTGCCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTA100.18148820326678766No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG100.18148820326678766No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAG100.18148820326678766No Hit
TTCAAGAGATGAACAGGAACACCAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCG100.18148820326678766No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG100.18148820326678766No Hit
GTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCCACGAGA90.16333938294010888No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAG90.16333938294010888No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGG90.16333938294010888No Hit
GATGAACCCTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGCTGATGCT90.16333938294010888No Hit
TCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG90.16333938294010888No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA90.16333938294010888No Hit
AGTCTGGTGTTCCTGTTCATTCAAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC90.16333938294010888No Hit
GTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCG90.16333938294010888No Hit
CCTCCAGGTCGAATCTTCAAACGACGACGATCACGCGTCGCTCCAGTATTCCAGGGTTCATCAGATCCTACGGTA90.16333938294010888No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCA90.16333938294010888No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA80.1451905626134301No Hit
CTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGG80.1451905626134301No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAG80.1451905626134301No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG80.1451905626134301No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG80.1451905626134301No Hit
GGTGTTCCTGTTCATCTCTTGAATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT80.1451905626134301No Hit
TGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80.1451905626134301No Hit
TCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGG80.1451905626134301No Hit
AAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGA80.1451905626134301No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTCTGTCTCTTATACACATCTCCGAGCCCACG80.1451905626134301No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC80.1451905626134301No Hit
GTACCGTAGGATCTGATGAACCCTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAG80.1451905626134301No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGG80.1451905626134301No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT80.1451905626134301No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACG80.1451905626134301No Hit
AGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAT70.12704174228675136No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC70.12704174228675136No Hit
AGATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAAAA70.12704174228675136TruSeq Adapter, Index 10 (96% over 28bp)
CATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATC70.12704174228675136No Hit
GTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCG70.12704174228675136No Hit
AAAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT70.12704174228675136No Hit
AAAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCC70.12704174228675136No Hit
CCCTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGCTGATGCTCGTGCT70.12704174228675136No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAG70.12704174228675136No Hit
GAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC70.12704174228675136No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT70.12704174228675136No Hit
TTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC70.12704174228675136No Hit
GCTGATGCTCGTGCTTTCTTGGCAATAAGTACCGTAGGATCTGATGAACCCTGGAATACTGGAGCGACGCGTGAT60.10889292196007261No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA60.10889292196007261No Hit
GTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGA60.10889292196007261No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGC60.10889292196007261No Hit
GTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACC60.10889292196007261No Hit
TCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTG60.10889292196007261No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC60.10889292196007261No Hit
AAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGG60.10889292196007261No Hit
CTGGAGGCACGGCTGATGCTCGTGCTTTCTTGGCAATAAGTACCGTAGGATCTGATGAACCCTGGAATACTGGAG60.10889292196007261No Hit
CTGGTGTTCCTGTTCATTCAAGAGATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATC60.10889292196007261No Hit
AAAAAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCTGTCTCTTATACACATCTCCGAGCCCA60.10889292196007261No Hit
AAAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCCAC60.10889292196007261No Hit
CCTGTTCATTCAAGAGATGAACAGGAACACCAGACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGG60.10889292196007261No Hit
GGTGTTCCTGTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC60.10889292196007261No Hit
AAAGTCTGGTGTTCCTGTTCATCTCTTGAATGAACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC60.10889292196007261No Hit
AAAAAGTCTGGTGTTCCTGTTCATCTCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC60.10889292196007261No Hit
GTTCCTGTTCATCTCTTGAATGAACAGGAACACCACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC60.10889292196007261No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC60.10889292196007261No Hit
GTGTTCCTGTTCATCTCTTGAATGAACAGGAACACCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA60.10889292196007261No Hit
CTGGAATACTGGAGCGACGCGTGATCGTCGTCGTTTGAAGATTCGACCTGGAGGCACGGCTGATGCTCGTGCTTT60.10889292196007261No Hit
GTTCATTCAAGAGATGAACAGGAACACCAGACTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAG60.10889292196007261No Hit
AAGTCTGGTGTTCCTGTTCATCTCTTGAACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCT60.10889292196007261No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)