FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002294682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3123320
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGC175830.5629586465683951No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG109490.3505564591524403TruSeq Adapter, Index 3 (96% over 28bp)
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC69100.22123893805309736No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC68190.21832537171983657No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC53640.17174032760011781No Hit
GTAGTATTTAGCCTTACGAGATGGTCCTCGCAGATTCACACGGGATTCCTCGTGTCCCGTGCTACTCGGGATTCA50460.16155885403993187No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGC49800.15944571801800647No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG41370.13245520791977766RNA PCR Primer, Index 3 (96% over 28bp)
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAG41080.13152670875862865No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG40280.12896533176235545No Hit
GTACTGGTTCACTATCGGTCACACAGTAGTATTTAGCCTTACGAGATGGTCCTCGCAGATTCACACGGGATTCCT39100.12518730069285247No Hit
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG36140.11571020580664167No Hit
GGCGAATACTGCACCAGAGAAGGTAATAGTCCTGTAGTCGAAAGTGGCAAGATACTAGCTGAATCCCGAGTAGCA34410.11017122805220085No Hit
CAGTAGTATTTAGCCTTACGAGATGGTCCTCGCAGATTCACACGGGATTCCTCGTGTCCCGTGCTACTCGGGATT34100.10917869446614499No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC32870.10524057733437497No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG31480.10079018480335028RNA PCR Primer, Index 3 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)