FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002294715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520656
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG397312.6127539693395483No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCT110800.7286329057985501No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCG107630.7077866394503425No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT97540.6414336970360159No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGATATG89610.5892851506192064No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGC78980.5193811092055008No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGATATG78660.5172767542429056No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG75590.49708809882050903No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGC69280.45559284940183714No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTGACGCTGCCGACGATA64860.42652644648099247No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTGACGCT60770.39963015961532394No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACG59970.39436927220883616No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTGACGCTGCCGACG59110.3887138182468619No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACATC57170.3759561662861291No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGATA56510.37161593417577676No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCC56140.3691827737502762No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCAG55630.36582895802864024No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGATAT51020.33551309434875476No Hit
CCCCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGA48870.321374459443819No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATC48450.31861249355541293No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGACGATAT47430.3119048621121411No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCCTGTCTCTTATACACATCTGACGCTGCCG45220.2973716606517187No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCC45070.2963852442630023No Hit
GAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCG44050.2896776128197304No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCT43290.2846797697835671No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATC42660.280536820950958No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATC40130.26389926452794055No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGCTGCCGACGATATG37860.24897149651203165No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGCTGTCTCTTATACACATCTGACGCTGC37120.2441051756610305No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTGTCTCTTATACACATCTGACGCTGCCGACG36920.24278995380940857No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACGATATG36620.24081712103197567No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACCTGTCTCTTATACACATCTGACGCTGCCGACGAT36440.23963342136551596No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACG35060.23055839058932462No Hit
CCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACG34860.22924316873770265No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTG34810.2289143632747972No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACAT32940.2166170389621321No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCA32570.21418387853663154No Hit
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTG32130.2112903904630633No Hit
CCTGGAAGCAGAGAGACTGCTTGTATAGTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATA32060.21083006281499567No Hit
GTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGACGAT31480.20701591944529202No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCTGTCTCTTATACACATCTGACGCTGCCGA31400.20648983070464325No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGCTGTCTCTTATACACATCTGACGC31310.20589798087141337No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACG30860.20293873170526402No Hit
GCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGAC30460.2003082880020202No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCCTGTCTCTTATACACATCTGACGCTGCCGAC30310.19932187161330372No Hit
CTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCA30230.19879578287265495No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCCTGTCTCTTATACACATCTGACGCTGCCGA29820.19609957807682998No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGCTGTCTCTTATACAC29350.19300880672551846No Hit
GAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTGACGCTGCCGA28730.18893161898549046No Hit
GGTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACTATACAAGCAGTCTCTCTGCTTCCA28650.1884055302448417No Hit
AGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGA28270.18590660872676001No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGCCGACGATATGC27840.1830788817457729No Hit
CAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGC27560.18123757115350217No Hit
CACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGC26390.17354352332151385No Hit
GTACATAAATCTACTGCTGCACTGCCGCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTG24960.16413968708241705No Hit
CCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACAC24280.1596679327869025No Hit
CAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACAT23940.15743205563914522No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCT23870.15697172799107753No Hit
GTATCAACGCAGAGTACATGGGCGGCAGTGCAGCAGTAGATTTATGTACCTGTCTCTTATACACATCTGACGCTG23850.15684020580591534No Hit
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGATATGC23680.1557222672320367No Hit
ACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCGACGATA23490.15447280647299585No Hit
TATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGCT23110.1519738849549142No Hit
GTATCAACGCAGAGTACATGGGGGTGTGGGTCTTGTCGCAGCTCTTGGGCTCGCTTCCGCTCTGGAAGTACAGGT23070.1517108405845898No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGACTGTCTCTTA22410.14737060847423744No Hit
GTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGTGAGATTTTGTGCTG21680.14257004871581738No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCCTGTCTCTTATACACATCTGACGCTGCC20410.13421838995801813No Hit
TATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCGA20290.13342925684704496No Hit
ACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTGACGCT20070.13198251281026083No Hit
ATGCTGGAGGTCGACCAGATGTCCGAAAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20050.13185099062509864No Hit
CCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCG19470.12803684725539505No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTCTGTCTCTTATACACATCTGACGCT19390.12751075851474628No Hit
CACAAAATCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTG19380.12744499742216517No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCAGACCCTGTCTCTTATACA19340.1271819530518408No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGCTGTCTCTTATACACATCTGACGCTG19310.12698466977409748No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTCTGTCTCTTATACACATCTGACGCTGCCGACGATA19300.1269189086815164No Hit
GAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCC19270.1267216254037731No Hit
GACAGTCGCTACGGGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACC18940.12455150934859692No Hit
GAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCTGTCTCTTATACACATCTGACGCTGCCGACGATA18910.12435422607085364No Hit
GTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCAGTG18680.12284172094148842No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGCCGA18060.11876453320146041No Hit
GAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCAGTG18050.11869877210887933No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCAGTG18040.11863301101629822No Hit
GAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCCTGTCTCTTATACACATCTGACGCTGCCGACGATA17760.11679170042402753No Hit
GCTGGAGGTCGACCAGATGTCCGAAAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17720.11652865605370315No Hit
GTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCG17630.11593680622047327No Hit
ACGCAGAGTACATGGGGAGGAGGCCGAAAGACCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCAGTGT17590.11567376185014888No Hit
ATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCCTGTCTCTTATACACATCTGACGCTGCCGAC17520.1152134342020812No Hit
GTACATGGGGAGGAGGCCGAAAGACAGAGGCTCCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCAGTG17280.1136351679801349No Hit
GGTCTGCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACA17160.11284603486916173No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGCTGTCTCTTATACACATCTGACGCTGCCGACGATATGC17130.11264875159141845No Hit
GCTCGGAACCGAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTGA16910.1112020075546343No Hit
GGTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTGTCTCTTATACACATCTGACGCTGCCGAC16850.11080744099914773No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGCTGTCTCTTATACACATCTGACGCTGCCGACGATA16420.10797971401816059No Hit
CCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGAC16370.1076509085552551No Hit
GAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTGACGCTGCCGAC16270.10699329762944414No Hit
CTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGACGATATGC16240.10679601435170083No Hit
GTATCAACGCAGAGTACATGGGGGTAACGTCAATTGCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCA16230.10673025325911975No Hit
GTCTTTCGGCCTCCTCCCCATGTACTCTGCGTCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCAGTGT16180.10640144779621426No Hit
GGTATCAACGCAGAGTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGTGGCTGTCTCTTATACA15990.10515198703717342No Hit
GTACATGGGAGGTTACAAGTTCCAGACTATCTCTGTGTGAATTGCTGTCTCTTATACACATCTGACGCTGCCGAC15960.10495470375943014No Hit
GTCCGAAAGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15820.1040340484632948No Hit
GAGCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTGACGCTGCCGA15820.1040340484632948No Hit
ACACAATGCCATTCATTTTTACACAAAATCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCA15770.1037052430003893No Hit
CCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTC15670.10304763207457834No Hit
ATCAACGCAGAGTACATGGGGAGGAGGCCGAAAGACAGAGGCTCGGTTCCGAGCCTGTCTCTTATACACATCTGA15670.10304763207457834No Hit
TCTCACCACAATTCACACAGAGATAGTCTGGAACTTGTAACCTCCCATGTACTCTGCGTTGATACCTGTCTCTTA15670.10304763207457834No Hit
CTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGATA15660.10298187098199724No Hit
CAATTGACGTTACCCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGATATGCA15550.10225849896360517No Hit
GTATCAACGCAGAGTACATGGGCAGTAATACGACTCACTATAGGGAGCTGTCTCTTATACACATCTGACGCTGCC15310.10068023274165887Clontech Universal Primer Mix Short (95% over 21bp)
GCCTCTGTCTTTCGGCCTCCTCCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGA15250.10028566618617228No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)