FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002294728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG214.285714285714285No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC17.142857142857142No Hit
AAATCAGCCTCTTTATTCTCAGCATTGACAACAGCCACCACAGCAATAAGCTGGAGGAACGCATTGACTACCTGA17.142857142857142No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTACCGGCCCC17.142857142857142No Hit
AACTGTAGCTTACACTGACTCCGAGCCCACGAGACGTTGGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA17.142857142857142RNA PCR Primer, Index 37 (96% over 29bp)
ACATCAGCCTCTTTATTCTCAGCATTGACCAAAGCCACCGCAGCAATACGCTGGAGGACCTCATTGACTACCTGA17.142857142857142No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCACTCAGCACCCTTTCAACAACTCCTGTT17.142857142857142No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGTTATCAGCCAAGT17.142857142857142No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATG17.142857142857142No Hit
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG17.142857142857142No Hit
AACTGTAGCTTACACTGACTCCGTGGGACAGTTTCTCCCCAATGAGACGACCGTCTGTGTTGTTATCAGCCAAGC17.142857142857142No Hit
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCAAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA17.142857142857142No Hit
GAAGCAGTGGATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGATTTAAAAAAAAAAAAGA17.142857142857142Illumina PCR Primer Index 8 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)