FastQCFastQC Report
Wed 31 Aug 2022
EGAF00002294730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGACCTCCTTGGTAACTGTCGGTCTGTCCGCTCTTAACGGCCAC112.5No Hit
GTAAAAGTTGAAATGTCAGGGCCAGTCACAGTTTTGACTAGACAAACCACTGCTGCAGCACTTGATAGCCACACC112.5No Hit
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCTCTGTGGATTTGCCAATTTCAGCTCCATTGGGATCATG112.5No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGCCGTTCATTCATCTCGTATGCCGTCTT112.5RNA PCR Primer, Index 45 (100% over 23bp)
TACTTTTAGCCAAGCACATTTCCGAGCCCACGAGACGTAGAGTCATCTCGTATGCCGTCTTCTGCTTGAAAAACA112.5Illumina PCR Primer Index 5 (96% over 28bp)
CAAGCAATCAAGGCCAGTCTACCCTGCAGCCCTTGCCGCCTTCCCATAAGCAGCACTCTGCACAGCATCATCCAT112.5No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTGCATTCATCTCGTATGCCGTCTT112.5RNA PCR Primer, Index 45 (95% over 23bp)
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGNGAAGGGGCCCTG112.5No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)