FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCT59.25925925925926TruSeq Adapter, Index 7 (95% over 23bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT35.555555555555555TruSeq Adapter, Index 7 (95% over 21bp)
GCACAGGTCGTATACGCATTGGCGGAAGTACTCAGAGGGACTCAGCACCGCCTGGCAGGTCGCGAAGGGGCCCTG23.7037037037037033No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCAAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT23.7037037037037033RNA PCR Primer, Index 16 (95% over 22bp)
ACATCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCAGCGACCATCGACCATCTCGTATGCCGT11.8518518518518516No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCAGAGCCCACTAGACCATCGACCATCTCATCTGCCGTCTT11.8518518518518516No Hit
ACATCAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCAGCTCGTATGCCG11.8518518518518516No Hit
CAACCAAAATAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT11.8518518518518516TruSeq Adapter, Index 7 (95% over 21bp)
CAACCAAAAGAACAGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT11.8518518518518516TruSeq Adapter, Index 7 (95% over 21bp)
CTTCTTGTTTTCAGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC11.8518518518518516No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCACCTCGTATGCCGTCTT11.8518518518518516No Hit
CAACCAAAAGAACAGCCTATCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT11.8518518518518516TruSeq Adapter, Index 7 (95% over 21bp)
CAAGCAACAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT11.8518518518518516TruSeq Adapter, Index 7 (95% over 21bp)
CTTCAGTGGAACATTCAGTTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGANCCACTCGTATGC11.8518518518518516No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGNATGCCGTCTTCT11.8518518518518516RNA PCR Primer, Index 14 (95% over 21bp)
TGGAATAAGTTCACTCTCAAACTTCACAGGCTGTCTCTTATACACATCTCCGAGCCCACGCGACCATCGACCATC11.8518518518518516No Hit
CAACCAAAAGAATAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATAGACCAACTCGTATGCCGTCTT11.8518518518518516No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTATT11.8518518518518516No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCAATCAGCACCCTTTCAACAACTCCTGTT11.8518518518518516No Hit
CAAGCAATAAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACAAGCTCGNATGCCGTCTTCT11.8518518518518516No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGATACCATCGACAATCTCGCAGGCCGTCTTCT11.8518518518518516No Hit
CAAGCAAAAGAACAGCCTGTCTCTTAGACACATCTCCGAGCACACGAGACAATCGACCATCTNGAATGCAGTCTT11.8518518518518516No Hit
GGCTGTGGTGTGAGTCCGGGGGGCGGCCGTAGCGCGCCATTTGCACCCGCAGCTCGCGGCCGTCCAGCACGGCCC11.8518518518518516No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATATNGTATGCCGTCTT11.8518518518518516No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTG11.8518518518518516RNA PCR Primer, Index 16 (96% over 25bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCATATGCAGTCTT11.8518518518518516No Hit
CTTTGACAGCATCACGAGTGTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTCGGAAGAAGTAGG11.8518518518518516No Hit
GAAGCAGTTGATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAACAAAAAAACCCA11.8518518518518516TruSeq Adapter, Index 27 (96% over 28bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATATCGTATGCCGTCTT11.8518518518518516No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT11.8518518518518516TruSeq Adapter, Index 7 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTTTATACACATGTCCGAGCCCACGAGACCATCGACCAGCTCGTATGCCGTCTTC11.8518518518518516No Hit
CAACCACAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATCGACCAGCTCGTATGCCGTCTT11.8518518518518516No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCATCTT11.8518518518518516No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTGTT11.8518518518518516No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATCGACCATATCGTATGCCGTCTT11.8518518518518516No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCTTCTT11.8518518518518516No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCATCTACCATCTCGTATGCCGTCTTAT11.8518518518518516RNA PCR Primer, Index 14 (95% over 21bp)
AACTGTAGCTTACACATCTCCGCGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA11.8518518518518516RNA PCR Primer, Index 7 (96% over 28bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCAGCTCGTATGCCGACTTCT11.8518518518518516No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCAGAGCCCACGAGACCATCGACCAGCTCGTATGCAGTCTT11.8518518518518516No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCGCGTATGCCGTCCT11.8518518518518516No Hit
AACTGTAGCTTACACTGACTCCGTGGAACAGTTTCTCCCCAATGAGACGAACGTCTGTGTTGNTATCAGCCAAGC11.8518518518518516No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATATCCGAGCCCACGAGACCATCGACCATCGCGTATGCCGGCTT11.8518518518518516No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACTAGACCATCGACCATCTCGTATGCCGTCTT11.8518518518518516TruSeq Adapter, Index 7 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCATCAAACAACTCGTATGCCGGCTT11.8518518518518516No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGCGCCCACGAGACCATCGACCATCTCGTATGCCGTCTTCT11.8518518518518516TruSeq Adapter, Index 7 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)