FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002294737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123
Sequences flagged as poor quality0
Sequence length75
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTT1512.195121951219512Illumina Paired End PCR Primer 2 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCT75.691056910569105Illumina Paired End PCR Primer 2 (95% over 23bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGAACTCGTATGCCGTCTT32.4390243902439024No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTT32.4390243902439024Illumina Paired End PCR Primer 2 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACTAGACAACCAACGATCTCGTATGCCGTCTT21.6260162601626018Illumina Paired End PCR Primer 2 (95% over 21bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACAATCTCCGAGCCCACGAGACAACCAACGAGCTCGTATGCCGTCTTC10.8130081300813009Illumina Single End Adapter 1 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACGTCTCCGAGCCCACGAGACACCCAACGATCTCTTATGCCGTCTTCT10.8130081300813009Illumina PCR Primer Index 1 (95% over 22bp)
CAAGCAATCAAGGCCAGCCTTTCTCTTATACACATCTCCGAGCCAACGAGACAACCAACGATCTCGTATGCCGTC10.8130081300813009RNA PCR Primer, Index 36 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGAGCTCGTATGCCGTCAT10.8130081300813009No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAAGGAGCTCGTATGCCGTCTT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCCAACGATCTCGTATGCCGTCTT10.8130081300813009RNA PCR Primer, Index 36 (96% over 26bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATTCCTTCTT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCTTCTT10.8130081300813009No Hit
AAACTCCCTGACCAATCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTT10.8130081300813009Illumina Paired End PCR Primer 2 (95% over 21bp)
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGCCAACGAACTCGTATGCCGTCTT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGACCACGAGACAACCAACGATCTCGTATGCCGTATT10.8130081300813009No Hit
CTTCTTGTTTTCGGGACATGCTTCAGCCCCTAGCCCTCCTTGGTCACTGTCGCTCTGTCCACTCTTAACGGCCCC10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTACGAGCCCACGAGACAACCAACGATCTCGTATGCAGTCTT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCAACGAGACAACCAACGATCTCGTATGCCGTCTT10.8130081300813009RNA PCR Primer, Index 36 (95% over 23bp)
AAACCAAAAGAACAGCCTGTCTCTTATACACATCTCAGAGCACACAAGAGAACGAACGATATCGTATGCAGTCGG10.8130081300813009No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCTAGCCCACGAGACAACCAACTATCTCGTATGCCGTCTTCT10.8130081300813009RNA PCR Primer, Index 38 (95% over 23bp)
ACATCAGCCTCTTTATTCTCAGCATTGACAAAAGCCACCGCAGCAATAAGCTGGAGGACCGCATTGACTACCTGA10.8130081300813009No Hit
CAAGCAAAAGAACAGTCTGTCTCTTATACACATCTCCGAGCCCACTAGACAACCAACGATCTCGTATGCAGTCTT10.8130081300813009No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCCCGAGACAACCAACGATCTNGTATGCCGTCTT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTTTACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTT10.8130081300813009RNA PCR Primer, Index 36 (95% over 23bp)
CAAGCAAAAGAACAGCCTATCTCTTATACAAATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTT10.8130081300813009Illumina Paired End PCR Primer 2 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGACACAACCAACGAGCTCGTATGCCGTCTTCT10.8130081300813009Illumina Single End Adapter 1 (95% over 22bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTAGTATGCCGTCTT10.8130081300813009No Hit
CAAGCAATCAAGGCCAGTATCTTATACACATCTCCTAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCT10.8130081300813009Illumina PCR Primer Index 1 (95% over 23bp)
CAACCCAAAGAACAGCCTGTCTCTTATACCCATCTCCGAGCCCACGAGACAACCAACGATCTCTTATGCCGTCTT10.8130081300813009No Hit
CAACCAAAAGAAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGAGCTCGTATGCCGTCTT10.8130081300813009No Hit
CAAGCAATCAAGGCCAGTATCTTATATACATCTCCGAGCCCACGAGACAACCAACGAAGTCGTATGCCGTCTGCG10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCACACGAGACAACCAACGATCACGTATGCCGTCTT10.8130081300813009No Hit
CCTTTAACAAGTATGCCTGTCAGCACCACACTGGTGGCCACTTCTGCCAGCAGCACCCTTTCAACAACTCCTGTT10.8130081300813009No Hit
CAAGCACTCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCT10.8130081300813009Illumina Paired End PCR Primer 2 (95% over 23bp)
TTTCAGGTCAGAGCTGAAGTCCTCACGACGTTTGCCCGCTGTGGATTTGCCACTTTCAGCGCCATTGGGATCATG10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCGCACGAGACAACCAACGATCTCGTATGCCGGCTT10.8130081300813009No Hit
CAAGCAATCAAGGCCAGTCTCTTATACAAATCTCCGAGCCCACGATACAACCAACGTTATCTTATGCCTTCTCCT10.8130081300813009No Hit
GTTTCCAGGTCAAGGGTAACACAAACAAGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGA10.8130081300813009No Hit
CAAGCAAAAGAACAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGAACTCGGATGCCGTCTT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTAGACACATCTCCGAGCCCACGAGACAACCAACGAACACGTATGCAGTCGT10.8130081300813009No Hit
TAAAATAGTAGCCCTCCCGCACTCACCAGGTCCGGTCGGTAGTACCTGTGCACCACTTCTGCCCCTGCGCACATG10.8130081300813009No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGAAATCGTATGCCGTCTTCT10.8130081300813009No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGACCACGAGACAACCAACGATATCGTATGCCGTCTT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAACAACGATCTCGTATGCCGTCTT10.8130081300813009RNA PCR Primer, Index 36 (95% over 22bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAACCCACGATATCGTATGCCGTCTTCT10.8130081300813009Illumina PCR Primer Index 1 (95% over 23bp)
CAACCAAAAGAACAGCCGGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGAGCTCGTATACCGTCTT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACAAACGATCGCGTAGGCAGTCTT10.8130081300813009No Hit
CAGTGACATTATTCCCCCTCTTACCTTAAGGCTGTGATTCTTTCTCTCTTCTTTTTTCTTCCTGGCAGTATTATT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTNGTATGCCGTCTT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGACGTCTT10.8130081300813009No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCAACGAGACAACAAACGATATCGTATGCCGTCTTCT10.8130081300813009Illumina PCR Primer Index 1 (95% over 22bp)
CAAGCAAAAGAACAGCCTGTCTCTTTAACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCT10.8130081300813009RNA PCR Primer, Index 36 (96% over 25bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCACACGAGACAACCAACGATCTCGTATGCCGTCTTCT10.8130081300813009RNA PCR Primer, Index 36 (96% over 25bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTAT10.8130081300813009TruSeq Adapter, Index 1 (95% over 22bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCGCGTATGCCGGCTA10.8130081300813009No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACCACCCACGATCTCGTATGCCGTCTTAT10.8130081300813009TruSeq Adapter, Index 1 (95% over 23bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGAGCTCGTATGCCGTCTTCT10.8130081300813009Illumina Single End Adapter 1 (95% over 22bp)
GACACTTGGCCTGGAGTACGGTGATGGCGTTGAGGCTGGGGTAGGGCCGGGGCACCAGCAGCGGGTAGCCGGCAA10.8130081300813009No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTNGTATGCCGTCTT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAAACAACCAACGATCTCGTATGCCGTCAT10.8130081300813009RNA PCR Primer, Index 36 (95% over 21bp)
CAAGCAATCAAGGCCAGTCTCTTATACACCTCTCCGAGCCCACGAGACAACCAACGATCTCTTATGCCTTCTTCT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATATCGTATGCCGTCTT10.8130081300813009No Hit
GTAAAAGTTGAAAGCAGGTATGAAGCTGTCTCTTATACACATCTCCGAGCACACGAGACAACTAACGAACTCGTA10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGAACTCGTATGCCGACTT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGCCAACCAACGCTCTCGTATGCCGTTTT10.8130081300813009No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA10.8130081300813009Illumina PCR Primer Index 1 (96% over 28bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGATCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCT10.8130081300813009RNA PCR Primer, Index 36 (96% over 25bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTACGAGCCCACGAGACAACCAACGATCTCGTCTGCCGTCTT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTATCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTT10.8130081300813009Illumina Paired End PCR Primer 2 (95% over 21bp)
CTTTGACAGCATCACGAGTTTCACCGGGATCACCAATGCCATACTCATTTTCAGCAAACACTNGGAAGAAGTAGG10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGAACTCGTATGCCGACTG10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCTAGCCCACGAGACAACCAACGATCTCGTCTTCCGTCTT10.8130081300813009No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCGCGTGTGCCGTCTTAT10.8130081300813009No Hit
CAAGCAATCAAGGCCAGTCTACACATCTCCGAGCCCACGAGACAACCAACGAGCTCGTATGCCGTCTTCTGCTTG10.8130081300813009Illumina Single End Adapter 1 (96% over 27bp)
CCCCCCCCCTCCCCGCCTTTCTCTTCTCCACATCTCCGAGCCCACGAGACAACCAACGAGCTCGTATGCCGTCTT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGAAATCGTATGCCGTCTT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTACGAGCCCACGAGACAACCAACGACCTCGTATGCCGTCCC10.8130081300813009No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGCGCCCACGAGACAACCAACGATCTCTTATGCCTTCTTCT10.8130081300813009No Hit
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGATACAACCAACGATCTCGTATGCCGTCTT10.8130081300813009RNA PCR Primer, Index 36 (95% over 23bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGAACTCGTATGCCGTCTTCT10.8130081300813009TruSeq Adapter, Index 1 (95% over 22bp)
GTTTCCAGGTCAAGGGTAACGGAGCGTGGACCCAGTCCCGCGTCTCCTCCCGGGTGCGGGGGGCCCCCTCAGTGA10.8130081300813009No Hit
CAAGCAATCAAGGCCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTC10.8130081300813009No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGNATGCCGTCTTCT10.8130081300813009TruSeq Adapter, Index 1 (95% over 22bp)
CAACCAAAAGCACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTT10.8130081300813009Illumina Paired End PCR Primer 2 (95% over 21bp)
CAACCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACTAGACAACCAACGATATCGTATGCCGTCTT10.8130081300813009No Hit
CAACCAAAAGAACCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACCCAACGATCTCGTATGCCGTCTT10.8130081300813009RNA PCR Primer, Index 36 (96% over 26bp)
CAAGCAATCAAGGCCAGTCTCTTATACAAATATCCGAGCCCACGAGACAAACAACGATCTCGTATGACGTCTTCT10.8130081300813009Illumina PCR Primer Index 1 (95% over 22bp)
ACATCAGCCTCTTTATTCTCAGCATTGACAACAGCCACCGCAGCAATAAGCTGGAGGACCGCATTGACTACCTGA10.8130081300813009No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCCGAGACCACGAGACAACCAACGAGCGCGTATGCCGGCTT10.8130081300813009No Hit
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCATCTTCT10.8130081300813009TruSeq Adapter, Index 1 (95% over 22bp)
CAAGCAATCAAGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATATCGTATGCCGTATTCGG10.8130081300813009No Hit
AACTGTAGCTTACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTCTGCCTGAAAAAAAAA10.8130081300813009RNA PCR Primer, Index 1 (96% over 27bp)
CAACCAAAAGAACAGCCTGTCTCTTAACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTTC10.8130081300813009Illumina Paired End PCR Primer 2 (95% over 22bp)
CAAGCAATCAAGGCCAGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGAGATCGTATGCAGTCTTCT10.8130081300813009No Hit
CAAGCAAAAGAACAGCCTGTCTCTTATACACATCTCGGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTT10.8130081300813009Illumina Paired End PCR Primer 2 (95% over 21bp)
CAAGCAAACGAACAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAACCAACGATCTCGTATGCCGTCTT10.8130081300813009Illumina Paired End PCR Primer 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)