FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002294762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002294762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456417
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGC308956.769029199175315No Hit
CTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG105062.301842394126424TruSeq Adapter, Index 1 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG73561.6116840520839497No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGC70161.5371907707206347No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG65551.4361866451074348No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCA64151.405512941016658No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTC63091.382288565062213No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG60791.331896051198794No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTC58351.2784361669262976No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG52701.1546458611313777RNA PCR Primer, Index 1 (96% over 28bp)
GTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCA52081.1410617921768909No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCAC49571.0860682226998555No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTC44020.9644688957685625No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA40630.8901947122916105No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATG40550.888441929200709No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTC34530.7565450016103694No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAA34300.7515057502240275No Hit
ACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG33310.7298150594741213RNA PCR Primer, Index 1 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC28510.6246480740200299No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACG25560.5600141975430363No Hit
GTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGA24080.5275877103613581TruSeq Adapter, Index 1 (96% over 28bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA21750.4765379028388513No Hit
GGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCT21520.47149865145250947No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC20990.45988646347528683No Hit
CATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGG20660.4526562332253181TruSeq Adapter, Index 1 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG20530.44980796070260315TruSeq Adapter, Index 1 (96% over 28bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCA18740.41058943904368156No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACG18420.40357830668007544No Hit
CGTCAGATGTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC17910.3924043144755782No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG17770.3893369440665006No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA17190.37662926665746455No Hit
CACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG16270.35647226111209707TruSeq Adapter, Index 1 (96% over 28bp)
CGTCAGATGTGTATAAGAGACAGGTCCAGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT15420.33784894077126837No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACG15070.3301805147485742No Hit
GTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT14920.3268940464531339No Hit
GTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT14560.319006522544077No Hit
CGTCAGATGTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC14480.3172537394531755No Hit
GGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATG14430.31615825002136205No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT14320.31374817327137244No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACAT14220.31155719440774554No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA13910.3047651599305022No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACATCTCCGAGCCCAC13670.29950681065779755No Hit
CGTCAGATGTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC13580.29753492968053336No Hit
GACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCG13390.29337206983964226No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA13250.2903046994305646No Hit
GTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG13000.28482725227149736No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCA12930.2832935670669585No Hit
GTCCAGGGTGGACATGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTC12760.2795689029987928No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCT12690.27803521779425394No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA12100.26510844249885523No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACAC11910.2609455826579641No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT11640.25502993972617144No Hit
GTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCA11580.25371535240799536No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG11560.2532771566352699No Hit
GTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT11490.2517434714307311No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCCTGTCTCTTATACACATCTCCGAGCC11470.25130527565800576No Hit
GTCCAGGGTGGACACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCT11390.2495524925671042TruSeq Adapter, Index 1 (95% over 23bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG10060.2204124736808664No Hit
GTGTATAAGAGACAGCATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA9760.21383953708998568No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT9490.20792389415819307No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAG9470.20748569838546768No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG9400.20595201318092884TruSeq Adapter, Index 1 (96% over 28bp)
ATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA9340.20463742586275271No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCC9300.20376103431730194No Hit
GTGTATAAGAGACAGCATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA9140.2002554681354989No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGC9130.20003637024913623No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT8950.19609260829460778No Hit
AAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCC8760.19192974845371666No Hit
GTGTATAAGAGACAGCCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA8660.18973876959008976No Hit
GGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTG8180.17922207104468063TruSeq Adapter, Index 1 (95% over 24bp)
CCCGATGAGCAGCACTACCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT8140.17834567949922986No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGC8060.17659289640832834No Hit
GTCCAGGGTGGACATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTT7960.17440191754470144TruSeq Adapter, Index 1 (95% over 21bp)
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA7780.17045815559017302No Hit
GGTCGGTAGTACCTGTACGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATC7760.17001995981744764No Hit
GTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTC7740.16958176404472228TruSeq Adapter, Index 1 (95% over 22bp)
CGTCAGATGTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC7450.16322792534020422No Hit
CGTCAGATGTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATC7440.16300882745384154No Hit
AGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTC7160.15687408663568622No Hit
GTGTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATG7020.15380671622660858No Hit
GGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA7010.15358761834024587No Hit
CGTCAGATGTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTC6910.15139663947661897No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGCTGTCTCTTATACACATCTCCGAGCCCACGA6790.14876746484026668No Hit
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCCTGTCTCTTATACACATCTCCGAGCCCACGAG6740.14767197540845323No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA6680.1463573880902771No Hit
CCCGATGAGCAGCACTACCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTATACACATCTCCGAGCCCACGA6670.14613829020391442No Hit
TCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTT6580.1441664092266502TruSeq Adapter, Index 1 (95% over 21bp)
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA6420.14066084304484716No Hit
CCCGATGAGCAGCACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTC6290.13781257052213217TruSeq Adapter, Index 1 (95% over 22bp)
GTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATATCGTATGC6220.13627888531759333No Hit
GTGTATAAGAGACAGGTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCA5950.1303632423858007No Hit
GTCCAGGGTGGACATGGGCACGCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTA5920.12970594872671262No Hit
GTGTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCC5830.12773406774944843No Hit
GTTTCCAGGTCAAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGCCCA5780.12663857831763495No Hit
GTCCAGGGTGGACATGGGCACGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATG5760.1262003825449096No Hit
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC5670.12422850156764537No Hit
GATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACG5620.12313301213583193No Hit
GTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGC5540.12138022904493041No Hit
CGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTC5380.11787466286312737No Hit
CATCCATAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGT5370.11765556497676467No Hit
GTCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTGAAAAA5180.11349270513587356TruSeq Adapter, Index 1 (96% over 28bp)
GTATAAGAGACAGCGTGCCCATGTCCACCCTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCA5150.11283541147678547No Hit
CATCCGCAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT5080.11130172627224666No Hit
GTCCAGGGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGTCTTCTGCTTG5040.11042533472679589TruSeq Adapter, Index 1 (96% over 28bp)
CATCCGCAGCGTCCTCAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGCCGT5000.10954894318134514No Hit
CATCCATAGCGTCCTCAGCTGTCTCTTATACACATCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT5000.10954894318134514No Hit
GTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGC4990.10932984529498245No Hit
GGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACACATCTCCGAGCCC4980.10911074740861974No Hit
GTATAAGAGACAGAAAGTTGAAATGTCAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCA4890.10713886643135553No Hit
GTCAGATGTGTATAAGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGA4870.10670067065863015No Hit
GTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAAT4860.10648157277226747No Hit
GCGTCAGATGTGTATAAGAGACAGGTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCAC4830.1058242791131794No Hit
GTCCAGGGTGGGCATGGGCACGCTGTCTCTTATACACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACG4760.10429059390864057No Hit
AGAGACAGGTCCAGGGTGGACATGGGCACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATC4710.1031951044768271No Hit
GTGTATAAGAGACAGCGTGCCCATGCCCACCCTGGACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAT4660.10209961504501366No Hit
GTGTATAAGAGACAGGGTCACGTCCTTCTTGATCTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACA4610.1010041256132002No Hit
GTCCAGGGTGGACATGGGCACACTGTCTCTTATACACATCTCCGAGCCCACGAGACGATGCACAATCTCGTATGC4600.10078502772683752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)